During the recruitment period, approximately 120 obese children aged 8 to 11 years were attended to at the outpatient clinic, and of these, approximately selleck products 90 met the inclusion criteria. The head researcher was contacted by 77 parents of children
who showed interest in participating in the program. Of these, 32 children studied in the morning and 45 in the afternoon. Due to logistical reasons, the program was held in the afternoon. Thus, children who studied in the morning were allocated to the case group (intervention participants) (n = 32), and those studying in afternoon were allocated to the control group, respecting the pairing for gender and age (n = 45). Losses that occurred between the initial contact and the beginning
of the program totaled ten in the case group and 23 in the control group. Thus, each group initially consisted of 22 children, totaling 44 matched obese children. Children in the control group did not participate in the intervention; however, they maintained the conventional treatment (monitoring and traditional medical treatment). All children were instructed to maintain their usual activities during the study period and were advised by the hospital medical staff regarding the practice of physical activity selleck chemical and nutritional guidance during follow-up. This study is part of a larger study,17 which used a clinically significant difference in systolic blood pressure of 15 mmHg
and a standard deviation of 15 mmHg in the population of obese children to calculate the sample size, with type I error of 5% and type II error of 20% (pilot study), as this is the most important risk factor and the one that determines early cardiovascular consequences in childhood and adolescence.18 Considering these parameters, the minimum Protein kinase N1 sample size was 16 subjects in each group. To this number, 25% were added for potential losses and refusals, which coincided with the number of children that participated until the end of the study.17 After the start of the program, the following exclusion criteria were used: children from the case group who did not attend at least 90% of sessions, whether or not these sessions were regular, 19 and/or those whose parents or guardians did not participate in the nutrition guideline sessions; children from the control group whose pairs from the case group failed to participate in the intervention or were excluded from analysis. All children underwent health-related anthropometric, demographic, clinical, and quality of life assessments that were self-reported in the hospital during the morning, from 7:30 AM to 12 PM, from one week before to one week after the start and end of program. To characterize the sample, a questionnaire on sociodemographic and clinical aspects, which was completed by the child’s parent or guardian, was applied.