Feeder Llig into two groups, n Namely Cinacalcet AMG-073 the diabetic and diabetic / treatment with curcumin at a dose of 100mg/kg K Body weight / day of Bedieneroberfl Surface. Curcumin was dissolved in 1% gum arabic St. Each animal in Group D have again U Vehicle 1% gum arabic. Drugs and vehicle were t Administered daily by oral gavage for 8wk. Before sacrifice, rats were placed in individual metabolic K Provisional for 24 h urine collections for measuring creatinine and urine protein levels were determined by the Bradford method and Jaffe, respectively. 2.3 Sch Tzung the biochemical parameters of blood samples by cardiac puncture in each animal at the time of death in EDTA Vacutainer R Hrchen collected. EDTA blood was centrifuged at 3000 g, 41C for 15 for the extraction of plasma. The plasma was stored at 801c, were carried out to test. The plasma was used for the determination of glucose, urea and creatinine. 2.4 Measurement of lipid peroxidation of lipid peroxidation was evaluated by measuring the Thiobarbiturs Acid reactivity t of malondialdehyde, an end product of the fat Acid peroxidation. To this end, renal tissue was rinsed, weighed, resuspended at 50 mg / ml in physiological saline Solution, homogenized, and using the Thiobarbiturs Acid reacting substances assay kit. centrifuged at 8000 rpm, 41C for 15 min in accordance with the provisions in total GPx assay kit.
The oxidation of NADPH at NADP1 was the decrease in absorbance measured at 340 nm. 2.6 The histopathological analysis of the kidney was decapsulated. The H half The kidney was immediately snap-frozen in liquid nitrogen for protein extraction and then Border enzymatic assays. The remaining kidney was excised, cut cut into approximately 2 mm thick slices and fixed transversely in 10% formalin. After being embedded in paraffin, several transverse sections of kidney and have been found Rbt with H Matoxylin and eosin and periodic acid-Schiff for histological evaluation and found rbt With Azan Mallory will also demonstrate fibrosis in renal tissue. 0, normal, mild, 1, 2, and 3 moderate, severe: the H frequency and severity of lesions in the kidneys were quantitatively analyzed by light microscopy in the H half assessed with the following notes. The criteria for kidney damage are ending Of the thickening of the glomerular re degrees, interstitial fibrosis, arteriolopahty, hyaline casts and tubular degeneration. 2.7 Immunohistochemistry for collagen type IV, fibronectin, and VEGFR-II in formalin, paraffin-embedded tissue sections were used for immunohistochemical kidney F Staining. After dewaxing and hydration, were Objekttr hunter in saline Trisbuffered washed solution. Endogenous peroxidase activity t was determined by incubation of Objekttr hunter in methanol and 0.3% H2O2 in methanol inhibited. After overnight incubation with the primary Ren Antique Body, polyclonal rabbit anti-collagen IV monoclonal anti-flk-1 and fibronectin mouse monoclonal antibody Body, diluted 1:50, were at 41C, the Objekttr hunters washed in TBS and then HRP-conjugated goat anti-rabbit-HRP conjugated secondary- Ren goat anti-mouse secondary rantik body was added and incubated at room temperature for 45 minutes. The Objekttr hunter were washed in TBS and matoxylin with diaminobenzidine as the substrate, then disadvantages with H. A contr Without the negative prim Ren Antique been Body in the experiment included to validate the SPE Antique Body.
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