coli and Streptomyces . Gene 1997, 190:315–317.PubMedCrossRef 49. Janssen GR, Bibb MJ: Derivatives of pUC18 that have Bgl II sites flanking a modified multiple cloning site and that retain the

ability to identify recombinant clones by visual screening of Escherichia coli colonies. Gene 1993,124(1):133–134.PubMedCrossRef 50. Bierman M, Logan R, O’Brien K, Seno ET, Rao RN, Schoner BE: Tideglusib purchase Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. Gene 1992,116(1):43–49.PubMedCrossRef 51. Gregory MA, Till R, Smith MC: Integration site for Streptomyces phage selleck products phiBT1 and development of site-specific integrating vectors. J Bacteriol 2003,185(17):5320–5323.PubMedCentralPubMedCrossRef 52. Huang J, Lih CJ, Pan KH, Cohen SN: Global analysis of growth phase responsive gene expression and regulation of antibiotic biosynthetic pathways in Streptomyces coelicolor using DNA microarrays. Genes Dev 2001,15(23):3183–3192.PubMedCrossRef 53. Redenbach M, Kieser HM, Denapaite D, Eichner A, Cullum selleck compound J, Kinashi H, Hopwood DA: A set of ordered cosmids and a detailed genetic and physical map of the 8 Mb Streptomyces coelicolor A3(2) chromosome. Mol Microbiol 1996,21(1):77–96.PubMedCrossRef 54. R: A language and environment for statistical computing. http://​www.​R-project.​org

55. Gentleman RC, Carey VJ, Bates DM, Bolstad B, Dettling M, Dudoit S, Ellis B, Gautier L, Ge Y, Gentry J, et al.: Bioconductor: open software development for computational biology and bioinformatics. Genome Biol 2004,5(10):R80.PubMedCentralPubMedCrossRef 56. Smyth GK: Limma: Cediranib (AZD2171) linear models for microarray data. In Bioinformatics and Computational Biology Solutions using R and Bioconductor. Edited by: Gentleman R, Carey V, Dudoit S, Irizarry R, Huber W. New York: Springer; 2005:397–420.CrossRef 57. Smyth GK, Speed TP: Normalization of cDNA microarray data. Methods 2003, 31:265–273.PubMedCrossRef 58. Flärdh K, Leibovitz E, Buttner MJ, Chater KF: Generation

of a non-sporulating strain of Streptomyces coelicolor A3(2) by the manipulation of a developmentally controlled ftsZ promoter. Mol Microbiol 2000,38(4):737–749.PubMedCrossRef 59. Flärdh K: Essential role of DivIVA in polar growth and morphogenesis in Streptomyces coelicolor A3(2). Mol Microbiol 2003,49(6):1523–1536.PubMedCrossRef Competing interests The authors declare that they have no competing interests. Authors’ contributions PS prepared all biological material for the array experiment, and carried out the array hybridizations and data analyses together with GB, EL, and CPS, who contributed materials, technology and knowhow for the transcriptome experiments. EL contributed particularly to the bioinformatic analyses. PS also carried out the qRT-PCR and S1 nuclease protection assays.