Figure 4 Effect of single amino acid substitutions in E protein on the transport of VLPs. HUVEC were exposed to mutant VLPs. After 24 h, media at the lower chamber were collected and subjected to IFU assay. (A) Transport of mutant 6-LP VLPs. *Dasatinib price represents p < 0.01 (versus 6-LP). (B) Transport of mutant Eg VLPs. * and ** represent p < 0.01 and p < 0.05, respectively (versus Eg). The graphs show the mean of three determinations. The error bars show SD. The results are representative of 2 independent experiments. The combination of Ser 156 and Val 159 is important for the transport

of 6-LP VLPs From the result of Fig 4B, the transport of Eg P156 S did not increase. This finding suggests the possibility that the combination of amino acids at the position of 156 and 159 might

affect the transport of VLPs. To assess this hypothesis, we generated double mutants, 6-LP S156P V159I and Eg P156 S I159V VX-809 research buy (Table 1). As shown in Fig. 5, the transport of 6-LP S156P V159I was greatly reduced (p < 0.01; versus 6-LP VLPs) to the level of wild type Eg VLPs. The transport of Eg P156 S I159V was greatly increased (p < 0.01; versus Eg VLPs) to the level of wild type 6-LP VLPs. These results suggest that the combination of Ser 156 and Val 159 is important for the transport of 6-LP VLPs across HUVEC. Figure 5 Effect of double amino acid substitutions of E protein on the transport of VLPs. HUVEC were exposed to 6-LP, 6-LP S156P V159I, Eg Verteporfin P156 S I159V or Eg VLPs. After 24 h, media at the lower chamber were collected and subjected to IFU assay. * p < 0.01 (versus 6-LP). The graphs show the mean of three determinations. Fossariinae The error bars show SD. The results are representative of 2 independent experiments. Combination of amino acid sequence at 156 and 159 does not affect the N-linked glycosylation of E protein From the results of Figs. 4 and 5, we speculated that the combination of amino acid sequence at 156 and 159 might affect N-linked glycosylation at the position 154 resulting in unglycosylation of E protein of Eg P156 S. To assess this possibility, we analyzed the glycosylation of E protein in 6-LP VLPs, Eg VLPs,

6-LP S156P, Eg P156 S, 6-LP V159I, Eg I159V, 6-LP S156P V159I and Eg P156 S I159V. Western blotting of E protein showed the band of wild type 6-LP strain was higher than that of Eg strain (Fig. 6. lanes 2 and 3) because of glycosylation. E protein of 6-LP S156P, Eg I159V and 6-LP S156P V159I was unglycosylated (Fig. 6. lanes 4, 7 and 8), whereas E protein of 6-LP V159I and Eg P156 S I159V was glycosylated (Fig. 6. lanes 6 and 9). Interestingly, E protein of Eg P156 S was also glycosylated (Fig. 6. lane 5). These results suggest that the combination of the residues 156 and 159 does not affect the N-linked glycosylation and that glycosylation of E protein is not the determinant of the transport of VLPs.