Above pH 8, the acid-alkaline transition due to the deprotonation of the water ligand was observed. The produced thiolate/OH -coordinated ferric-P450st was stable at room temperature. The pK(a) value of 8.7 for the transition reflects the protonation properties of the distal side of the heme. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights LY2090314 reserved.”
“A critical stage in malaria transmission occurs in the Anopheles mosquito

midgut, when the malaria parasite, Plasmodium, ingested with blood, first makes contact with the gut epithelial surface. To understand the response mechanisms within the midgut environment, including those influenced by resident microbiota against Plasmodium, we focus on a midgut bacteria species’ intra-specific variation that confers diversity to the mosquito’s competency for malaria transmission. Serratia marcescens isolated

from either laboratory-reared mosquitoes or wild populations in Burkina Faso shows great phenotypic variation in its cellular and structural features. Importantly, this variation is directly correlated with its ability to inhibit Plasmodium development within the mosquito midgut. Furthermore, this anti-Plasmodium function conferred by Serratia marcescens requires increased expression of the flagellum MK-2206 datasheet biosynthetic pathway that is modulated by the motility master regulatory operon, flhDC. These findings point to new strategies for controlling malaria through genetic manipulation of midgut bacteria within the mosquito.”
“The Brugada syndrome is a well-known genetic disease comprising a distinct electrocardiographic pattern with a high risk for cardiac arrest. The Brugada electrocardiographic pattern has, however, been observed in other clinical conditions. We describe a case of hyperkalemia

presenting a Brugada type I pattern in the absence of typical electrocardiographic manifestations seen in hyperkalemia. J Cardiovasc Med 11:285-287 (C) 2010 Italian Federation of Cardiology.”
“Introduction: Listeria monocytogenes is the causative agent of listeriosis, a foodborne illness that affects mainly pregnant women, the elderly and immunocompromised HKI-272 patients. The primary treatment is a combination of ampicillin with an aminoglycoside, in addition to a second-choice drug represented by chloramphenicol, erythromycin, tetracycline and rifampicin. The aim of this study was to analyze the antimicrobial susceptibility profile of strains isolated from human sources in the last four decades. Methods: Sixty-eight strains were selected from the culture collection of the Laboratory of Bacterial Zoonoses/LABZOO/FIOCRUZ isolated in different regions of Brazil from 1970 to 2008 and primarily isolated from cerebrospinal fluid and blood culture. Susceptibility tests to antimicrobials drugs were evaluated using the criteria established by Soussy using the Kirby-Bauer method and E-Test strips were used to determine the minimum inhibitory concentration (MIC).