This selectivity was more robust when larger numbers of surrounding items and multiple features were included in the display, and it was absent when only a few items were presented immediately outside the CRF. In addition, the popout modulation
of V4 activity was eliminated when top-down attention was directed to locations outside of the CRFs during saccade preparation, indicating that the salience of popout stimuli is not sufficient AZD5363 solubility dmso to drive selection by V4 neurons. These results demonstrate that neurons in feature-selective cortex are influenced by bottom-up attention, but that this influence is limited by top-down attention.”
“Whether uni- or bilateral drainage should be performed for malignant hilar biliary obstruction remains a matter of debate. Moreover, endoscopic placement of bilateral metallic stents has been considered difficult and complicated. Although the Y-stent with a central wide-open mesh facilitates bilateral stent placement, it has limitations. This study evaluated the feasibility and efficacy of the Niti-S large Etomoxir in vivo cell D-type biliary stent (LCD) with a uniform large cell for both uni- and bilateral drainage of malignant hilar biliary obstruction.\n\nFrom April
2008 to March 2009, a total of 12 consecutive patients with unresectable malignant hilar biliary obstruction of Bismuth type 2 or greater underwent placement of LCD. Before LCD placement,
all the patients underwent endoscopic unilateral biliary drainage using a plastic stent or a nasobiliary drainage tube. If jaundice improved after the procedure, the plastic stent or nasobiliary drainage tube was replaced with the unilateral LCD. If jaundice did not resolve or contralateral cholangitis occurred, bilateral LCD placement was performed.\n\nSeven patients had unilateral and five patients had bilateral LCD placement. Technical LY2157299 success was achieved for all 12 patients. An early complication occurred for one patient (8%), and stent occlusion occurred for six patients (50%) because of tumor ingrowth (n = 4) or sludge (n = 2). These patients were managed by insertion of plastic stents (n = 4) or percutaneous transhepatic biliary drainage (n = 2). The median stent patency period was 202 days.\n\nThe newly designed endoscopic metallic stent may be feasible and effective for malignant hilar biliary obstruction, and endoscopic reintervention is relatively simple.”
“Objective: A major challenge in the management of patients with complex regional pain syndrome 1 is identifying those individuals who are at risk of developing severe problems. Data from large follow-up studies providing empirical evidence are largely lacking. The goal of this study was to obtain an expert-agreed priority list of parameters that are correlated with a poor prognosis.
a case series of 33 children with OAFNS ascertained from a comprehensive review of the literature and report an additional retrospective series of eight patients displaying features consistent with OAFNS. Notably, in a subset of our cases, we have observed abnormalities in nasal ossification and bony structures of the maxilla that have not previously described in OAFNS and are not seen in either FND or OAVS. We present the phenotype and novel naso-maxillary findings and explore potential etiologic and developmental pathways for OAFNS. click here We highlight the differences in phenotypic characteristics of OAFNS compared to OAVS and FND. These observations support the classification of OAFNS as a discrete syndrome. Further phenotypic refinements of OAFNS 3-Methyladenine ic50 are needed to understand pathogenesis of this syndrome and the newly described nasal malformation may help identify the etiology. (C) 2013 Wiley Periodicals, Inc.”
“The content of elements in rainwater is
an indirect indicator of its occurrence in air dust. This is sometimes referred to as rain fallout and is investigated in applied environmental pollution monitoring schemes. The annual content of elements in rainwater may be recognized as good index for assessing influence of those environmental factors on human body. The possible relationship between the concentrations of selected elements in rainwater and the frequency of hospitalization by reason
of angina pectoris, stroke, and peripheral venous thrombosis was investigated in the Opole Voivodship (Poland) area during the period 2000-2002. There is a relatively high or partly significant S3I-201 concentration correlation between frequency of hospitalization by reason of these conditions and content of lead, cadmium, chromium, zinc, and chloride in rainwater. Significant gender-dependent differences were observed only in peripheral venous thrombosis, where important correlations with lead, cadmium, and chromium were found only in men.”
“A variety of components have been isolated from various higher plants and characterized as allelochemicals, which can play an important role in natural plant communities. Leukamenin E is an ent-kaurene diterpenoid isolated from Isodon racemosa (Hemsl) Hara. Phospholipase D (PLD) is a key enzyme involved in membrane phospholipid catabolism during plant growth, development, and stress responses. To further explore and elaborate the responses of PLD to leukamenin E treatment, the activities and expression patterns of the PLD gene in Arabidopsis thaliana (A. thaliana) callus were researched. When A. thaliana callus was incubated with leukamenin E at concentrations of 100 and 200 mu M for 48 h, the activities of PLD in microsomal and mitochondrial membranes exhibited an upregulation behavior, with the highest levels at 24 and 36 h, respectively.
However, the anti-inflammatory potential of hispidin still remains uncharacterized. RESULTSIn this study, the effects of hispidin on activation PF-00299804 cell line of nuclear factor kappa B (NF-B) and the subsequent production of inducible nitric oxide synthase (iNOS) were determined in the lipopolysaccharide (LPS)-induced macrophage RAW 264.7 cells. Our data indicated that hispidin inhibits transcriptional activity of NF-B
in a dose-dependent manner. Hispidin also attenuated LPS-induced NF-B nuclear translocation and associated inhibitor of kappa B (IB-) degradation. Furthermore, hispidin deceased iNOS protein expression and the generation of reactive oxygen species (ROS) in the LPS-induced cells, but did not affect phosphorylation of mitogen-activated protein kinases. CONCLUSIONThese findings suggest that hispidin exhibits anti-inflammatory activity through suppressing ROS mediated NF-B pathway in mouse macrophage cells. (c) 2014 Society of Chemical Industry”
“Background: Adenomectomy is the treatment of choice for ACTH-secreting
adenomas. Although the development of ACTH deficiency immediately after adenomectomy suggests surgical success, disease recurrence was reported in patients who developed hypocortisolism postoperatively. In the current study, we examined the value of measuring perioperative plasma ACTH and cortisol levels in predicting disease recurrence of patients with ACTH-secreting adenomas.\n\nMethods: Consecutive patients (n = 55; 41 Bafilomycin A1 supplier females, 14 males) with clinical, biochemical, Nepicastat and histological documentation of ACTH-secreting adenomas were investigated after pituitary adenomectomy. All patients were followed with clinical monitoring and frequent measurements of plasma ACTH and serum cortisol levels, and none received glucocorticoids
unless or until they developed symptoms of adrenal insufficiency or when their serum cortisol levels were <= 3 mu g/dL.\n\nResults: Postoperative serum cortisol levels reached <= 3 mu g/dL in 46 of 55 and were >= 4 mu g/dL in the remaining 9. Simultaneously measured plasma ACTH levels in the latter 9 patients were >40 ng/L when the serum cortisol reached its nadir. In contrast, among the 46 patients who had serum cortisol levels of <= 3 mu g/dL, plasma ACTH levels measured simultaneously were <= 20 ng/L in 38 of 46 and >20 ng/L in the remaining 8. During a mean follow-up period of nearly 7 years, patients who had a nadir plasma ACTH of >20 ng/L developed recurrences even though their postoperative serum cortisol levels were <= 3 mu g/dL.\n\nConclusions: Despite profound hypocortisolemia after adenomectomy, a simultaneously measured plasma ACTH level of >20 ng/L in the perioperative period is highly predictive of future recurrence of ACTH-secreting adenomas.
However, our knowledge about the chain of molecular and cellular events translating stress experience into altered behavior is still rather scant. Here, we have characterized a murine ortholog Selisistat of the putative tumor suppressor gene DRR1 as a unique stress-induced
protein in brain. It binds to actin, promotes bundling and stabilization of actin filaments, and impacts on actin-dependent neurite outgrowth. Endogenous DRR1 localizes to some, but not all, synapses, with preference for the presynaptic region. Hippocampal virus-mediated enhancement of DRR1 expression reduced spine density, diminished the probability of synaptic glutamate release, and altered cognitive performance. DRR1 emerges as a protein to link stress with actin dynamics, which in addition is able to act on synaptic function and cognition.”
“Ethanol and aqueous extracts of the different parts of Piper sarmentosum were analysed by HPLC for marker compounds to standardise these extracts. The standardised extracts were investigated for antioxidant activity (-carotene linoleate model and DPPH model), anti-TB activity (microplate tetrazolium assay), and estimation of total phenolic and amide contents. The extracts of the different parts exhibited different antioxidant activity, phenolic and amide contents (p 0.01). The ethanol extracts exhibited better antioxidant activity as compared to the
aqueous extracts. The leaf ethanol extract was further investigated for Screening Library dose response relationship and its EC50 was found to be 38 g mL-1. All the extracts have exhibited anti-TB activity with MIC/MBC 12.5 g mL-1. The leaf methanol extract was fractionated and the ethyl acetate fraction exhibited anti-TB activity with MIC/MBC 3.12 g mL-1 while MIC/MBC of isoniazid (INH) was found to be 0.5 g mL-1. A positive correlation was found between antioxidant activity and total polyphenols, flavonoids and amides, in the -carotene check details linoleate model (p = 0.05) and in the DPPH model (p = 0.01). The analytical method was found to have linearity 0.9922, coefficient of variance 5% and accuracy 95.5 5 to 96.9
5. This plant possesses promising antioxidant as well as anti-TB properties.”
“Non-line-of-sight (NLOS) propagation degrades the performance of wireless location systems. Thus, developing algorithms that are robust to NLOS considerations is of great importance. Based on time-of-arrival (TOA) and angle-of-arrival (AOA) measurements, this paper introduces a new approach, which consists of incorporating the coordinates of dominant scatterers as unknowns in the location algorithm. It is assumed that the first arriving path signal at each base station (BS) experiences a single dominant scatterer, but the BSs are allowed to have different dominant scatterers. Locating the mobile station is accomplished by means of a nonlinear optimization procedure under nonlinear constraints.
A46 binds to MyD88, Mal/TIRAP, TRIF and TRAM and suppresses the activation of NF-kappa B and interferon regulatory factors. Each of these cytosolic adaptors selleck kinase inhibitor has a TIR domain that is critical for oligomerization during signaling. Although the structure of A46 is unknown, it has alternatively been described as an alpha/beta-fold TIR domain, or an all alpha-helical Bcl-2 fold. Here we provide experimental evidence that the C-terminus of A46 adopts a dimeric alpha-helical structure, and that this segment retains the ability to interact with monomeric Mal. Furthermore, a peptide fragment
of A46 termed VIPER, previously shown to retain the biological properties of the full-length protein, does not interact with Mal in vitro. In summary, we provide for the first time a biophysical analysis of the binding of a poxvirus protein to a TIR domain-containing Vactosertib adaptor molecule. (C) 2011 Elsevier Ltd. All rights reserved.”
“This study aimed to identify the optimal neural progenitor cell transplantation time for spinal cord injury in rats via the subarachnoid space. Cultured neural progenitor cells from 14-day embryonic rats, constitutively expressing enhanced green fluorescence protein, or media alone, were injected into the subarachnoid space of adult rats at 1 hour (acute stage),
7 days (subacute stage) and 28 days (chronic stage) after contusive spinal cord injury. Results showed that grafted neural progenitor cells migrated and aggregated around the blood vessels of the injured region, and infiltrated the spinal cord parenchyma along the tissue spaces in the acute stage transplantation group. However, this was not observed
in subacute and chronic stage transplantation groups. O4- and glial fibrillary acidic protein-positive cells, representing oligodendrocytes and astrocytes respectively, were detected in the core of the grafted cluster attached to the cauda equina pia surface in the chronic stage transplantation group 8 weeks after transplantation. Both acute and subacute stage transplantation groups were negative for O4 and glial fibrillary acidic protein cells. Basso, Beattie and Bresnahan scale score comparisons indicated that rat hind limb locomotor activity showed better recovery after acute stage QNZ solubility dmso transplantation than after subacute and chronic transplantation. Our experimental findings suggest that the subarachnoid route could be useful for transplantation of neural progenitor cells at the acute stage of spinal cord injury. Although grafted cells survived only for a short time and did not differentiate into astrocytes or neurons, they were able to reach the parenchyma of the injured spinal cord and improve neurological function in rats. Transplantation efficacy was enhanced at the acute stage in comparison with subacute and chronic stages.
\n\nMain methods: Animals were fed an ethanol liquid diet or isocaloric control diet for 5 weeks. Isolated perfused rat livers were preserved in Histidine-Tryptophan-Ketoglutarate at 4 degrees C. After 24 h of storage, livers were subjected to 120 min of reperfusion with Krebs-Henseleit bicarbonate buffer at 37 degrees C. Animals were pre-treated with cobalt protoporphyrin (CoPP, 5 mg/kg, i.p.) or zinc protoporphyrin (ZnPP,
25 mg/kg, i.p.), HO-1 inducer and antagonist, respectively.\n\nKey findings: In the model of ischemia/isolated perfusion, endogenous HO-I was downregulated in the livers fed with ethanol diet (ED I/R). In ED I/R group, portal pressure and lactate dehydrogenase release were significantly increased, while bile output and hyaluronic acid clearance GSK2879552 solubility dmso decreased compared to rats fed on control diet (CD I/R). Furthermore, hepatic glutathione content decreased and lipid peroxidation increased
in the ED I/R group compared to the CD I/R group. These alterations were attenuated by upregulation of HO-1 with CoPP pretreatment.\n\nSignificance: Our results suggest that chronic ethanol consumption aggravates hepatic injury during cold I/R and it is likely due to downregulation of endogenous HO-1. Prior induction of HO-1 expression may provide a new strategy to protect livers against hepatic I/R injury or to increase the donor transplant pool
through modulation of marginal find more alcoholic steatotic livers. (c) 2011 Elsevier Inc. All rights reserved.”
“Global epidemic studies have suggested that coffee consumption is reversely correlated with the incidence Vorinostat mw of type 2 diabetes mellitus (T2DM), a metabolic disease. The misfolding of human islet amyloid polypeptide (hIAPP) is regarded as one of the causative factors of T2DM. Coffee extracts have three major active components: caffeine, caffeic acid (CA), and chlorogenic acid (CGA). In this study, the effects of these major coffee components, as well as dihydrocaffeic acid (DHCA) (a major metabolite of CGA and CA), on the amyloidogenicity of hIAPP were investigated by thioflavin-T based fluorescence emission, transmission electronic microscopy, circular dichroism, light-induced cross-linking, dynamic light scattering, and MTT-based cell viability assays. The results suggest that all components show varied inhibitory effects on the formation of toxic hIAPP amyloids, in which CA shows the highest potency in delaying the conformational transition of the hIAPP molecule with the most prolonged lag time, whereas caffeine shows the lowest potency. At a 5-fold excess molar ratio of compound to hIAPP, all coffee-derived compounds affect the secondary structures of incubated hIAPP as suggested by the circular dichroism spectra and CDPro deconvolution analysis.
“The apolipoprotein E gene (APOE) has been found to be associated with age-related macular degeneration (AMD). Reported associations have been questioned,
as they are opposite those for Alzheimer’s disease and cardiovascular disease. The authors examined associations between APOE genotype and AMD using a case-control study (2,287 cases and 2,287 controls individually matched on age, sex, and country of origin) nested within Melbourne Collaborative Cohort Study participants aged 48-86 years at AMD detection. The odds ratio for early AMD among participants with epsilon 2-containing genotypes (epsilon 2 epsilon 2/epsilon 2 epsilon 3/epsilon 2 epsilon 4) was 1.32 (95% confidence interval (CI): 1.11, 1.58; P = 0.002) versus persons with genotype epsilon 3 epsilon 3. Associations with early AMD varied by smoking status; AC220 chemical structure epsilon 2-containing genotypes were positively associated with early AMD for never and
previous smokers (never smokers: odds ratio (OR) = 1.40, 95% CI: 1.12, 1.76 (P = 0.003); previous smokers: OR = 1.39, 95% CI: 1.00, 1.93 (P = 0.05)) but not for current smokers (OR = 0.66, 95% GSK1838705A CI: 0.34, 1.30 (P = 0.2; interaction P = 0.05). The epsilon 4-containing genotype group (epsilon 3 epsilon 4/epsilon 4 epsilon 4) had an inverse association with early AMD among current smokers only (OR = 0.41, 95% CI: 0.22, 0.77 (P = 0.005)). These results highlight the importance of stratifying by smoking status in elderly populations. Smokers who survive to old age may be more likely to possess unknown click here genotypes which modify exposure-disease associations.”
“Background: The use of beta-blockers during the perioperative period remains controversial. Although
some studies have demonstrated their protective effects regarding postoperative cardiac complications, others have demonstrated increased mortality when beta-blockers were introduced before surgery.\n\nMethods: In this observational study involving 1,801 patients undergoing aortic reconstruction, we prospectively assessed beta-blocker therapy compared with no beta-blocker therapy, with regard to cardiac and noncardiac postoperative outcomes using a propensity score approach. The impact of beta-blockers was analyzed according to the intraoperative bleeding estimated by transfusion requirements.\n\nResults: In-hospital mortality was 2.5% (n = 45), beta-blocker use was associated with a reduced frequency of postoperative myocardial infarction (OR = 0.46, 95% CI [0.26; 0.80]) and myocardial necrosis (OR = 0.62, 95% CI [0.43; 0.88]) in all patients, but also with an increased frequency of multiple organ dysfunction syndromes (OR = 2.78, 95% CI [1.71; 4.61]). In patients with severe bleeding (n = 163; 9.1%), the frequency of in-hospital death (OR = 6.65, 95% CI [1.09; 129]) and/or multiple organ dysfunction syndromes (OR = 4.18, 95% CI [1.81; 10.
Epidemiological studies, clinical observations, laboratory analyses and immunoserological specific assays (indirect immunofluorescence, IIF, and ELISA) were performed. Food samples were analyzed by artificial digestion, and Trichinella
larvae isolates were identified to the species level by multiplex PCR. The main source of infection, commercially available food, had a parasite load of 1.1 muscle larvae per gram. Larvae were identified as Trichinella spiralis. Patients presented predominantly with oedema, fever and myalgia; and laboratory findings and/or immunoserological tests were positive for trichinellosis. Individuals received outpatient treatment. Selleckchem Androgen Receptor Antagonist No deaths or secondary sequelae were recorded. Results suggest
that the presence of T.-spiralis infection should be suspected in all endemic areas, especially where animal husbandry and official food safety controls are not properly conducted. The lack of the LY3039478 price cases reported ought not to be taken as a proof of parasite absence. We highlight the importance of the urgent need to implement interdisciplinary and inter-institutional programs aimed to control infection transmission, to guarantee food safety and to conduct epidemiological surveillance studies. (C) 2014 Elsevier B.V. All rights reserved.”
“ObjectiveThe aim of this study was to assess the feasibility of incorporating the Delphi process within the simplifying conditions method (SCM) described in elaboration theory (ET) to identify conditions impacting the complexity of procedural skills for novice learners. MethodsWe generated an initial list of conditions impacting the complexity of lumbar puncture (LP) from key informant interviews (n=5) and a literature
review. Eighteen clinician-educators from six different medical specialties were subsequently recruited as expert panellists. Over three Delphi rounds, these panellists rated: (i) their this website agreement with the inclusion of the simple version of the conditions in a representative (epitome’) training scenario, and (ii) how much the inverse (complex) version increases LP complexity for a novice. Cronbach’s -values were used to assess inter-rater agreement. ResultsAll panellists completed Rounds 1 and 2 of the survey and 17 completed Round 3. In Round 1, Cronbach’s -values were 0.89 and 0.94 for conditions that simplify and increase LP complexity, respectively; both values increased to 0.98 in Rounds 2 and 3. With the exception of high CSF (cerebral spinal fluid) pressure’, panellists agreed with the inclusion of all conditions in the simplest (epitome) training scenario. Panellists rated patient movement, spinal anatomy, patient cooperativeness, body habitus, and the presence or absence of an experienced assistant as having the greatest impact on the complexity of LP.
\n\nApproach: Small molecule library mouse A fresh
frozen femur of a 54 year old female was scanned under two different environments: in air and immersed in water (dry and wet CT). Thereafter, the proximal femur was quasi-statically loaded in vitro by a 1000 N load. The two QCT scans were manipulated to generate p-FE models that mimic the experimental conditions. We compared p-FE displacements and strains of the wet CT model to the dry CT model and to the experimental results. In addition, the material assignment strategy was reinvestigated. The inhomogeneous Young’s modulus was represented in the FE model using two different methods, directly extracted from the CT data and using continuous spatial functions as in Yosibash et al. [2007a. Reliable simulations of the human proximal femur by high-order finite element analysis validated by experimental observations. J. Biomechanics 40, 3688-3699].\n\nResults: Excellent agreement between dry and wet FE models was found for both displacements and strains, i.e. the method is insensitive to CT conditions and may be used in vivo. Good agreement was also found between FE LDN-193189 results and experimental observations.
The spatial functions representing Young’s modulus are local and do not influence strains and displacements prediction. Finally, the p-FE results of all three fresh frozen human femurs compare very well to experimental observations exemplifying that the presented method may be in a mature stage to be used in clinical computer-aided decision making. (C) 2008 Elsevier Ltd. All rights reserved.”
factor expression levels, which sensitively reflect cellular development and disease state, are typically monitored via cumbersome, reagent-intensive assays that require relatively large quantities of cells. Here, we demonstrate a simple, quantitative approach to their detection based on a simple, electrochemical sensing platform. This sensor sensitively Barasertib datasheet and quantitatively detects its target transcription factor in complex media (e.g., 250 mu g/mL crude nuclear extracts) in a convenient, low-reagent process requiring only 10 mu L of sample. Our approach thus appears a promising means of monitoring transcription factor levels.”
“Objective:\n\nTo characterize milnacipran effects on systolic blood pressure (SBP), diastolic blood pressure (DBP), and heart rate (HR) in fibromyalgia patients using 24-hour ambulatory blood pressure monitoring (ABPM).\n\nMethods:\n\nThis dose-escalation study included a 7-week double-blind treatment period and 2-week single-blind discontinuation period. Patients were randomized 2:1 to milnacipran (n=210) or placebo (n = 111), with 50% of patients classified as ‘hypertensive’ at baseline (SBP >= 130 mmHg, DBP >= 85 mmHg, or current antihypertensive medication). Analyses were conducted at Weeks 4 and 7, after milnacipran dosages were escalated to 100 and 200 mg/day, respectively.
However, their mechanisms of action are not fully elucidated. In this study, mechanisms of PBP-mediated antipromoting effects were investigated in a mouse model employing the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). Compared to controls, a single HIF cancer topical application of TPA to mouse skin increased the translocation of protein kinase C (PKC) from cytosol to membrane. Pretreatment with PBPs 1-3 decreased TPA-induced translocation of PKC isozymes (alpha, beta, eta, gamma, epsilon) from cytosol to membrane, whereas PBPs 4 and 5 were less effective. The levels of PKCs delta
and zeta in cytosol/membrane were similar in all the treatment groups. Complementary confocal microscopic evaluation showed a decrease in TPA-induced PKC alpha fluorescence in PBP-3-pretreated membranes, whereas pretreatment with PBP-5 did not show a similar decrease. Based on the experiments with specific enzyme inhibitors and phosphospecific antibodies, both PBP-3 and PBP-5 were observed to decrease TPA-induced level and/or activity of phosphatidylinositol 3-kinase (PI3K) and AKT1 (pS473). An additional Selleckchem EVP4593 ability of PBP-3 to inhibit site-specific phosphorylation of PKC alpha at all three positions responsible for its activation [PKC
alpha (pT497), PKC PAN (beta II pS660), PKC alpha/beta II (pT638/641)] and AKT1 at the Thr308 position, along with a decrease in TPA-induced PDK1 protein level, correlated with the inhibition of translocation of PKC, which may impart relatively stronger chemoprotective activity to PBP-3 than to PBP-5. Altogether, PBP-mediated decrease in TPA-induced PKC phosphorylation correlated well with decreased TPA-induced NF-kappa B phosphorylation and downstream target proteins associated with proliferation, selleck inhibitor apoptosis, and inflammation in mouse skin. Results suggest that the antipromoting effects of PBPs are due to modulation of TPA-induced PI3K-mediated signal transduction. (c) 2012 Elsevier Inc. All rights reserved.”
“PURPOSE. To identify the distribution, differential Toll-like receptor (TLR) expression, and functional contribution of monocyte subpopulations in the inflammatory stage of Eales’ disease (ED).\n\nMETHODS.
Peripheral blood mononuclear cells were isolated from nine patients during the inflammatory stage of ED and nine age-and sex-matched healthy controls. The expression of CD14, CD16, TLR-2, and TLR-4 on monocytes was measured by flow cytometry. The CD14(+), CD16(-), and CD16(+) monocyte populations were sorted on the basis of magnetic-activated cell-sorting methodology, and levels of cytokines were measured by ELISA.\n\nRESULTS. In ED patients, the number of circulating monocytes was significantly expanded compared with that in controls (P = 0.01), with a marked increase in the nonclassic CD16(+) subset, which showed an activated phenotype in patients that correlated with levels of serum proinflammatory cytokines and clinical progression. A higher expression of cell surface TLR-2 (P = 0.