This is consistent with the report that autophagosomes can be formed in the absence of intact regular microtubules, www.selleckchem.com/products/BAY-73-4506.html but at a significantly lower extent. After autophagosomes mature, they fuse with lysosomes to form autolysosomes. Lysosomes distribute throughout the cytoplasm through anterograde and retrograde move ment. Our results show that regular non acetylated microtubules seem to play no role in the process since their interruption did not cause accumulation of LC3II in the absence of lysosomal inhibitor. This indicates the pre sence of highly specific cytoskeletal elements are involved in the trafficking of autophagosomes and lysosomes involved in autophagy. HADC6 is a microtubular deacetylase and regulates microtubule stability.

Inhibition of HADC6 enhances microtubular acetylation leading to antero grade trafficking of lysosomes away from centrosomes in addition to an inhibition of autophagosomal biogen esis. Since microtubular acetylation causes the recruitment of the molecular motors dynein and kine sin 1 to microtubules, acetylated microtubules may serve for not only the kinesin dependent antero grade trafficking but also the dynein dependent retro grade trafficking of either lysosomes or autophagosomes. In addition to the opposite roles in polymerization depolymerization of regular microtubules by direct bind ing to b tubulin, paclitaxel and nocodazole have oppo site effects in the acetylation of a tubulin and stabilization of acetylated microtubules. Paclitaxel enhances, but nocodazole inhibits a tubulin acetylation and stabilization of acetylated microtubules.

However, both of them fail to block autophagosomal degradation. Both paclitaxel and vinblastine enhance the levels of a tubulin acetylation, but exhibit opposite effects on the polymerization of acetylated microtubules and also opposite roles in autophagosomal degradation. These results suggest that it is not the levels of acetylated a tubulin that affect autophagosomal degradation. Similar to paclitaxel, nocodazole does not damage the integrity of acetylated microtubules although the total levels of acetylated a tubulin are reduced. Vinblastine enhances the levels of acetylated a tubulin, but causes depolymerization of both regular and acetylated micro tubules. The treatment not only blocks fusion of LC3II assoiated autophagosomes with lysosomes, but also reduces efficiency of the LC3I to LC3II conversion simi lar to paclitaxel or nocodazole.

It seems that regular microtubules are involved in, but not essential for the conversion of LC3I to LC3II and degradation of LC3II while acetylated microtubules are required GSK-3 for trafficking of either mature autophagosomes or lysosomes. When autolysosomes were preserved by treatment with bafilo mycin A1, a dramatic decrease of number of autolyso somes was observed in cells treated with vinblastine.