, 1990 and Nieminen et al., 1994). It protected the hepatocytes against significant programmed cell death induced by MCT, demonstrating the important role of reducing levels of ATP in this process. The protection provided by DTT indicates that the oxidation of thiol groups is also involved in the induction of apoptosis by MCT. Thus, our results suggest that the metabolite-induced mitochondrial energetic impairment, together with a decrease of cellular glutathione and protein thiol groups, can contribute to the toxic effects of MCT on hepatocytes. None declared. This work was supported by grants from Fundação

de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Process number 2004/09882-7, Brazil. The authors thank Michele Costa Lima for technical assistance. “
“Solitary wasps are known to inject their venoms into insects or spiders, AZD1208 supplier paralyzing the prey in order to feed their larvae. Therefore, the solitary wasp venoms should contain a variety of neurotoxins acting on nervous systems. In fact, polyamine toxins (Eldefrawi et al., 1988), peptide neurotoxins (Yasuhara et al., 1987 and Konno et al., 1998) and a protein paralyzing toxin (Yamamoto et al., 2007) have so far been found in several solitary wasp venoms. Besides the neurotoxins, we have found that cytolytic peptides are also present in the solitary wasp venoms. Eumenine mastoparan-AF (EMP-AF) was the first to be found (Konno et al., 2000 and Santos

Cabrera et al., 2004), having similar characteristics tetracosactide to those of mastoparan, a representative of the cytolytic peptides RG7422 cell line in social wasp venoms. Eumenitin is also homologous to mastoparan, but has an extra hydrophilic amino acid at the C-terminus without amide modification (Konno et al., 2006). Anoplin was isolated from spider wasp venom and is the smallest molecule in this type of peptides (Konno et al.,

2001). Decoralin, another linear cationic α-helical peptide, has features similar to anoplin, but like EMP-AF vs. eumenitin, it has an extra hydrophilic amino acid without amide modification at the C-terminus ( Konno et al., 2007). Except for anoplin, these cytolytic peptides were found in solitary eumenine wasps, alternatively called “mud dauber wasps” or “potter wasps”, because they construct their pot-shaped nest with mud. Additionally, the eumenine wasps prey only on caterpillars, Lepidopteron larvae. In our continuing survey of biologically active substances in solitary wasp venoms, we have isolated four
ar cationic α-helical peptides from two species of the eumenine solitary wasps, Eumenes rubrofemoratus and Eumenes fraterculus. Two of them, named eumenitin-R and eumenitin-F, are highly homologous to eumenitin, whereas the other two, named eumenine mastoparan-ER (EMP-ER) and eumenine mastoparan-EF (EMP-EF), are similar to EMP-AF, and thus, can be classified as mastoparan peptides.