Images of the stained cells were obtained using a fluorescent microscope attached to a digital camera. Data are expressed as mean (±standard error of the mean, SEM) and analysed and presented using GraphPad Prism. Groups of two were analysed using Student’s t-test, groups of three or more were analysed using either one-way analysis of variance (ANOVA) with a Dunnets post-hoc test or, if multiple variables were involved, two-way ANOVA with Bonferroni post-hoc test was applied. Values were considered to be significantly different LGK-974 datasheet when p<0.05. The authors thank Professor Nancy Rothwell for support. The research was funded by the UK Department for Trade and Industry (A.P., N.J.A.),

the Biotechnology and Biological Sciences Research Council (UK) and Medical Research Council (UK) (R.A.S.), and Eisai Ltd. London (L.M.). “
“The cuneate nucleus (CN) receives and processes incoming somesthetic input from the primary afferents of the forelimb (Andersen et al., 1962, Andersen Cytoskeletal Signaling inhibitor et al., 1964a and Andersen et al., 1964b) before relaying this information, in part, to the ventral posterior nucleus (VPL) of the thalamus (Alloway and Aaron, 1996, Berkley et al., 1980, Kemplay and Webster, 1989 and Massopust et al., 1985). The organization of CN has been described in monkey (Florence et al., 1989), cat (Nyberg, 1988), raccoon (Rasmusson, 1989), and rat (Beck, 1981, Li et al., 2012, Maslany et al., 1990 and Nord, 1967), and it is generally agreed

that the rostrocaudally oriented CN is partitioned into rostral, middle, and caudal regions (Berkley et al., 1986, Bermejo et al., 2003, Dykes et al., 1982 and Maslany et al., 1992). Recently, the details of the somatotopic organization of CN in rat were elucidated using fine-grain electrophysiological mapping (Li et al., 2012). The middle region was further partitioned into medial, central, and lateral zones. The central zone containing cytochrome oxidase (CO)-stained

clusters, termed barrelettes, was mapped, and the individual labeled clusters were associated with the representation Oxymatrine of the glabrous forepaw digits and digit and palmar pads; the medial zone was mapped to the ulnar representation of the wrist, forearm, and upper arm, while the lateral zone was mapped to the radial representation of the wrist, forearm, and upper arm. A lateral tail region was identified that received input primarily from the shoulder, head/neck, and ear. This somatotopy in the forelimb-intact rat provided a useful starting point from which to compare CN reorganization following deafferentation. CN organization and the resulting reorganization in rat have been studied following limb amputation (Crockett et al., 1993 and Lane et al., 1995), dorsal rhizotomy (Sengelaub et al., 1997), and nerve transection (Crockett et al., 1993). Time of deafferentation has varied from embryonic (Killackey and Dawson, 1989 and Rhoades et al., 1993), neonatal (Lane et al., 1995 and Lane et al., 2008), and adult (Sengelaub et al.