Ed in the same after treatment with siRNA Calretinin for 24 hours or 48 hours or clones SV1 and SV8 with antisense oligonucleotides incubated indicate the position of the rods marker proteins Of 50 kDa and 25 kDa. B: expression were reduced by 80% in calretinin clone SV1 after exposure EX 527 SEN0014196 to ASO 300 nmol / L for 48 hours CR9. The average expression levels were still some hours Ago as the clones contr The M2 and M3. The relative values of the clones SV1 and SV8 and M2 and M3 of Western blot were as shown in Figure 4A, the clone SV8 was set at 100%. C: Quantitative analysis of the impact of the regulation by calretinin CR9 ASO down on crocidolite cytotoxicity T cells treated with NSO compared.
For the analysis of the OD values were converted to log, averaged over the repeated wells with the same clone and treatment in an experiment, and treated to an analysis of variance, with treatment, clone, and their interaction as factors and experiment as a block . Clone was treated as a Feeder Lliger factor, and so the treatment clone interaction Vargatef was used as the denominator in the F-test for treatment effect. For graphical means were back transformed and the differences are presented as percentage Ver alteration. The MTT signal was in the treated OSA CR9 four clones CR averaged reduced 11 to 26%, but not treated contr in the ASO CR9 The clones M2 and M3. D: siRNA treated clones was more variability in terms of t and repeated cloning attempts, but in terms of control NSO, the siRNA treatment consistently reduced the MTT signal of all four clones CR9 ASO treated CR 10 to 16%.
This effect was in M2 and M3 clones contr absent On. Prevent asbestos toxicity T calretinin 2331-16% owned erh Increase the reqs Susceptibility to toxicity T of crocidolite in the CR-clones. As for ASO, the effect of siRNA was in M2 and M3 clones contr absent Am. Combining the results of controlled cloning The M2 and M3 CR9 exposed to ASO or siRNA CR, the net effect compared to treatment with a contr The track was close to zero. This eliminates down-regulation of calretinin was primarily the Ph Phenotype of cells treated antisense clones CR protection again sensitive to the toxic effects of crocidolite. This is best CONFIRMS calretinin, s protective effect. Exposure of mesothelial cells to asbestos fibers activated signaling pathways in cell survival and cell death that participates, including the AKT pathway.
50 L Ngere exposure of human mesothelial cells to SV40 erh Ht of the cells survive through activation of AKT and conclude Lich induces a transformation loss of contact inhibition of cell regeneration and several foci.51 We determined the H he controlled comparison of the phosphorylation of AKT cell lines demonstrated transfected to four SV40 transfected clones and five clones calretinin, showed a high expression of calretinin. These cell-based enzyme-linked immunosorbent assay was observed both in basal conditions and after exposure to asbestos is carried out at short notice. The short-term asbestos has led to an increase in the ratio Ratio PACT / ACT by a factor of 1.5 was introduced, the rise Similar for all three types of clones. PACT basal values differ significantly from one clone to another, the difference between the lowest and was h Chsten value almost twice as high. But Ana