into cells with 50 nM of dexmedetomidine in control cells treated A how CONFIRMS by quantifying the intensity of t the F ERK staining p best. There was no significant difference between cells controlled Were treated LY2228820 in the cells with EGF receptor RTK inhibitor AG 1478 to 1 mM and cells with dexmedetomidine plus AG 1478th Phosphorylated ERK showed cytoplasmic F Staining, which was surrounded, but not the kernel. Similar results were ratio 0 1 2 3 4 *** 30 60 120 0 3 6 9 Time Ratio ** 30 60 120 Dex Time Control 30 min 60 min DMG Dex controlled Dex 236 bp 120 bp 659 bp 303 min 1 2 3 Figure 7 mRNA expression increased dexmedetomidine CFOs and ht FosB in astrocytes.
The cells were incubated for 30 min, 60 or 120, in the absence of a medicament or in the presence of 50 nM of dexmedetomidine. The Gr E of the PCR product of 659 bp cfos, the 303 bp and 236 bp FOSB TBP. Southern blot of a repr Sentative experiment. Similar Brivanib results were independent of three Ngigen experiments received. Average mRNA expression was quantified as the ratio Ratio between Fos and c TBP and TBP between FosB and. Mr. H.E. Values are indicated by vertical bars. * Indicates a statistically significant difference in the controlled group For CFOs and FosB analyzed by ANOVA followed by Fisher’s LSD test. Married Ratio controlled by 0 3 6 9 * A2 AG1478 AG1478 Dex Dex + 303 bp 236 bp 659 bp a1 configuration Dex Dex + AG1478 AG1478 ratio * a3 0 3 6 9 DMG AG1478 AG1478 Dex Dex + 0 0.5 1 1.
5 ratio Ratio of b2 * DMG U0126, U0126 Dex Dex + 303 BP 236 bp 659 bp b1 contr U0126, U0126 Dex Dex + 0 0.5 1 1.5 2 ratio Ratio b3 * DMG Dex Dex + U0126 U0126 Figure 8 mRNA expression induced dexmedetomidine CFOs and FosB EGF receptor and ERK action in astrocytes requires. The Gr E of the PCR product of 659 bp cfos, the 303 bp and 236 bp FOSB TBP. The cells were incubated for 60 min in the absence of a drug in the presence of 50 nM of dexmedetomidine of 1 mM AG 1478 or dexmedetomidine plus AG 1478 incubated. Southern blot of a repr Sentative experiment. Similar results were independent of three Ngigen experiments received. Average mRNA expression was detected as a ratio Quantified ratio between CFOs and FosB and between TBP and TBP. Mr. H.E. Values are indicated by vertical bars.
* Indicates a statistically significant contr The, AG 1478 or dexmedetomidine plus AG 1478 groups for CFOs and FosB analyzed by ANOVA followed by Fisher’s LSD test. The cells were incubated for 60 min in the absence of a drug in the presence of 50 nM of dexmedetomidine, 10 mM U0126, an inhibitor of ERK1 / 2 phosphorylation or dexmedetomidine and U0126 incubated. Southern blot of a repr Sentative experiment. Similar results were independent of three Ngigen experiments received. Average mRNA expression was detected as a ratio Quantified ratio between CFOs and FosB and between TBP and TBP. Mr. H.E. Values are indicated by vertical bars. * Indicates a statistically significant contr That, U0126 or U0126 plus dexmedetomidine groups for c-fos and FosB. EGF receptor transactivation in astrocytes 198 B Li et al British Journal of Pharmacology 154 191 203 after 10 min, 1 and 2 h incubation was observed. EGF-induced ERK1 / 2 phosphorylation, Western blot showed that 10 ng of EGF significantly ml_1 erh Increase of ERK1 / 2 phosphorylation in astrocytes after 20 minutes exposure. A band of 44 kDa representing ERK1 and ERK2 band of 42 kDa. Stimulation by EGF is sensitive to 1 mM AG in 1478, but not by 10 mM