Treatment of mouse or human hepatocytes with a farnesoid X receptor (FXR) www.selleckchem.com/B-Raf.html agonist GW4064 or bile acids induced hepatic Abcg5/g8 expression. A functional FXR binding site was identified in the Abcg5 gene promoter. Study of tissue-specific Fxr knockout mice demonstrated that loss of the Fxr gene in the liver attenuated bile acid induction of hepatic Abcg5/g8 and gallbladder cholesterol content, suggesting a role of FXR in the regulation of cholesterol transport. Conclusion: This study revealed a new mechanism by which increased Cyp7a1 activity

expands the hydrophobic bile acid pool, stimulating hepatic cholesterol synthesis and biliary cholesterol secretion without increasing intestinal cholesterol absorption. This study demonstrated that Cyp7a1 plays a critical role in maintaining cholesterol homeostasis and underscores the importance of bile acid signaling in regulating overall cholesterol homeostasis. (HEPATOLOGY 2011) The liver is a major organ involved in de novo cholesterol synthesis and catabolism, biliary cholesterol secretion, and reverse this website cholesterol transport. Cholesterol homeostasis in the liver is maintained by balancing de

novo cholesterol synthesis, uptake, and elimination. Biliary secretion of cholesterol, either in the form of free cholesterol or bile acids, is the only significant route for eliminating cholesterol in mammals.1 Cholesterol 7α-hydroxylase (cytochrome Carnitine palmitoyltransferase II P450 7A1 [CYP7A1]) is the rate-limiting enzyme in the bile acid biosynthetic pathway in the liver and thus controls cholesterol and bile acid homeostasis. Deficiency of CYP7A1 in humans is associated with hypercholesterolemia and premature atherosclerosis.2 Bile acids are not limited to being physiological detergents that facilitate intestinal fat, sterols, and fat-soluble vitamin absorption and distribution but also act as signaling molecules that activate

the farnesoid X receptor (FXR) and several cell signaling pathways to maintain lipid, glucose, and energy metabolism.1, 3 It has been reported that overexpression of CYP7A1 in mouse liver (Cyp7a1-tg mice) prevents lithogenic diet–induced atherosclerosis.4 We recently reported that Cyp7a1-tg mice are resistant to high-fat diet–induced obesity, insulin resistance and fatty liver, and maintained cholesterol, bile acid, and triglyceride homeostasis.5 The cholesterol-lowering effect of stimulation of bile acid synthesis has been attributed to increased conversion of cholesterol into bile acids and stimulation of low-density lipoprotein (LDL) receptor–mediated cholesterol uptake into the liver. Hepatic cholesterol is secreted into bile by a heterodimeric cholesterol efflux transporter, adenosine triphosphate–binding cassette G5/G8 (ABCG5/G8), in the canalicular membrane of hepatocytes.