HIRASHIO SHUMA1, NAKASHIMA AYUMU1, DOI SHIGEHIRO1, ANNO KUMIKO2, AOKI ERIKO2, SHIMAMOTO AKIRA2, YORIOKA NORIAKI3, KOHNO NOBUOKI4, MASAKI TAKAO1, TAHARA HIDETOSHI2 1Department of Nephrology, Hiroshima University Hospital, Hiroshima, Japan; 2Department of Cellular and Molecular Biology, PXD101 mw Graduate School

of Biomedical Science, Hiroshima University, Hiroshima, Japan; 3General Incorporated Association Hiroshima Kidney Organization, Hiroshima, Japan; 4Department of Molecular and Internal Medicine, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan Introduction: Telomeric G-tail is a key component to maintain total telomere structure of loop. Telomere shortening leads to progression of arteriosclerosis through the cellular senescence and in chronic kidney disease

patients. We investigated whether telomeric G-tail length could be used as a novel predictor for new-onset cardiovascular events in hemodialysis patients. Methods: We performed a prospective observational study involving a cohort of 203 Japanese hemodialysis patients. We measured G-tail length in peripheral blood mononuclear cells (PBMCs) in hemodialysis patients by using hybridization protection assay (HPA) and followed cardiovascular events during a median follow-up period of 48 months. The lengths of telomeric G-tails and total telomeres were also measured in control subjects without SB203580 solubility dmso chronic kidney disease who were matched for age and gender. Multiple logistic regression analysis was used to assess independent predictors of CVD history. Analyze of a future cardiovascular event was made with the Morin Hydrate Cox proportional hazard model. Results: G-tail was significantly shorter in hemodialysis

patients than that in control subjects. Although G-tail length was correlated with age in hemodialysis patients and control subjects, rate of decline per year of G-tail length in patients was more gradual than that in control subjects. Telomeric G-tails, but not total telomeres, were independently and negatively associated with clinical history of cardiovascular disease. During follow-up, 80 cardiovascular events occurred. Total telomere length did not predict cardiovascular events. However, the length of telomeric G-tails was associated with future cardiovascular events, which persisted after adjustment for multiple factors. Conclusion: Telomeric G-tail length is a good predictor of new-onset cardiovascular events in hemodialysis patients. ZHU BIN Institute of Clinical Medical Science, China-Japan Friendship Hospital Introduction: DNase I is the major nuclease found in body fluids such as serum and urine. In mammal, the pancreas and kidney exhibits the highest DNase I activity with nearly 60–65% of serum DNase I was secreted by pancreas.