The previous study had shown that GT inhibited human organic anion transporting polypeptides (OATPs). Moreover, Epigallocatechin-3-gallate (EGCG), a major catechins derivative, Sirolimus decreased P-glycoprotein (P-gp) and Breast Cancer Resistance Protein (BCRP) expressions
and functions. Our preliminary study demonstrated that GT could inhibit transport of [3H]MPP+ (1-methyl-4-phenylpyridinium), a prototypical organic cation, in rat renal slices. There is no evidence whether consumption of green tea during cationic drugs treatment interfere with drug efficiency and drug secretion. Therefore, the present study aimed to elucidate the interaction of GT and its catechins on a major renal basolateral organic cation transporter, OCT2. Methods: The uptake of [3H] https://www.selleckchem.com/products/VX-765.html MPP+
was measured in the second segment of the renal proximal tubule (S2) cells stably expressing human OCT2 (S2-hOCT2) and rat Oct2 (S2-rOct2) in the presence of GT and its catechins. The IC50 values of GT and catechins were determined. Results: GT and (-)epicatechin-3-gallate (ECG), but not EGCG inhibited the OCT2-mediated [3H]MPP+ transport with IC50 values higher than 1 mg/ml and 1 mM, respectively, in S2-hOCT2 and S2-rOct2. This IC50 values were higher than the plasma concentration of catechins in daily tea consumption. Conclusion: The weak interaction of GT and its catechins with renal organic cation transporter OCT2 indicates that consumption of green tea beverage or catechins supplements does not interfere PDK4 with therapeutic organic cationic drugs that secreted via OCT2 in kidney. MINATOGUCHI SHUN1,2, OZEKI TOSHIKAZU1,2, WARTANABE MITSURU1,2, MURAI YUKARI1,2, KAWATO RUI1,2, RYUGE AKIHIRO1,2, OZEKI TAKAYA1,2, KIDA TAKASHI1,2,
OYAMA YUKAKO1,2, HAMADA TAKUYA3, NOMURA ATSUSHI1,2, TOMINO TATSUHITO1,2, SHIMIZU HIDEAKI1,2, FUJITA YOSHIROU1,2 1Chubu-Rosai Hospital, Nephrology; 2Chubu-Rosai Hospital, Rheumatology; 3Chubu-Rosai Hospital, Internal Medicine Introduction: We report the case of myoglobin-induced acute kidney injury(AKI) caused by compartment syndrome(CS) after percutaneous cardiopulmonary support(PCPS) applied to deal with cardiac arrest secondary to acute myocardial infarction (AMI) and massive gastrointestinal hemorrhage due to cytomegalo-virus (CMV) colitis. Methods & Results: A 34-year-old man went into cardiac arrest due to AMI, and we conducted PCI and PCPS. The right external iliac artery was damaged by accident during cannulation and we performed massive blood transfusion and fluid infusion (more than 20 L) for treating the massive hemorrhage. On the day of admission, ischemic symptoms developed in his left lower limb after PCPS use. He was diagnosed as having CS of the left lower limb because of highly elevated compartment pressure. On the 2nd day, he had anuria caused by myoglobin-induced AKI and we started hemodialysis. On the 6th day, liver functions were abnormal. On the 13th day, massive melena developed and he required blood transfusions.