Nevertheless, the three administered groups were detected an obvious increase in the percentage of CD3+ and the ratio of CD3+/CD19+ without a dose-dependent relationship. The result of higher ratios of CD3+/CD19+ in all of the three carbon dot-treated groups indicated that the proliferation of T lymphocytes was more significant than that of B lymphocytes in peripheral lymphocytes under the administration of carbon dots, which coincided with the results of splenocyte proliferation. The two

major subpopulations of T lymphocytes are Th cells and Tc cells. In general, CD4+ cells act as helper cells and CD8+ cells act as cytotoxic cells. The Th cells can also be defined as two major functional subpopulations, Adriamycin mouse Th1 and Th2 cells. The Th1 response produces cytokines (IFN-γ, TNF-β, etc.) that support inflammation and activate mainly certain T cells and macrophages, whereas the Th2 response secretes cytokines (IL-4, IL-5, etc.) which activate

PI3K Inhibitor Library research buy mainly B cells and immune responses that depend upon antibodies [17, 18]. The Tc cells can recognize antigens combined with class I MHC in the click here presence of appropriate cytokines (IFN-γ) and give rise to cytotoxic T cells, which display cytotoxic ability. Several studies have addressed the influence of nanoparticles on Th1 and Th2 responses. It is reported that some small engineered nanoparticles such as 80- and 100-nm nanoemulsions, 95- and 112-nm PEG-PHDA nanoparticles, and 123-nm dendrosome, could induce the Th1 response [19]. We observed that carbon dots could promote the percentage of CD8+ and decrease the ration of CD4+/CD8+. Nevertheless, both the percentages of CD8+ and CD4+ had a significant increase without a dose-dependent relationship at 9 days after administration, and the ration of CD4+/CD8+ decreased only in the 2-mg/kg group. The levels of IFN-γ also had a significant increase in the carbon dot-treated

groups. From these results, we presume that the main modulator pathway of carbon dots was to activate the Th1 cells. The Th1 cells Adenosine secreted IFN-γ cytokines, which played an important role in the activation of the proliferation and differentiation of the Tc cells (CD8+ T cell), and then the percentage of CD8+ increased, and the ratios of CD4+/CD8+ declined. The IFN-γ cytokines could also be produced by Tc cells, which were dedicated to the increase of the levels of IFN-γ. On the other hand, the production of IL-4 cytokines was hardly to be detected both in the blood serum and the supernatant of induced lymphocytes, indicating that carbon dots, at the treated dose, could not induce the response of Th2 cells, which play an important role in the activation process of humoral immunity.