The protocol was Nirogacestat order approved by the institutional ethics committees and this study was carried out according to the principles of the Declaration of Helsinki and Good Clinical Practice guidelines. The eligibility criteria were histologically proven unresectable colorectal adenocarcinoma; adequate bone marrow, liver, and renal function; Eastern Cooperative Oncology Group (ECOG) performance status (PS) <2; age >20 years at the time of enrolment; and expected survival STAT inhibitor time >12 weeks. Any

previous chemotherapy (only 1 regimen was allowed) must have been completed at least 28 days before enrolment. Postoperative adjuvant therapy was not counted as prior chemotherapy. Patients with multiple malignancies, Vactosertib cell line comorbidities that could influence the outcome, prior radiotherapy, pregnancy or lactation, symptomatic peripheral neuropathy, or a history of serious drug hypersensitivity were excluded. Written informed consent was obtained from all of the subjects. Treatment schedule An implantable port and a disposable

pump were employed so that chemotherapy could be administered on an outpatient basis. An outline of the administration method for mFOLFOX6 therapy, in which the dose of oxaliplatin was reduced from 100 mg/m2 to 85 mg/m2, is shown in Figure 1. A 5-HT3 antagonist and a steroid were administered as premedication. A 2-hour intravenous infusion of oxaliplatin plus l-leucovorin was followed by bolus intravenous injection of 5-FU, after which 5-FU was administered by continuous infusion for 46 hours. An

oral steroid was administered for 3 days from day 2 after the start of therapy. The duration of one cycle was 2 weeks. Figure 1 Schedule for mFOLFOX Therapy. With each treatment cycle, administration was only started after confirming that all of the following criteria had been fulfilled. (1) Hematological toxicity: leukocyte count >3,000/mm3 Y-27632 research buy and platelet count >75,000/mm3.   (2) Non-hematological toxicity: Grade 2 or less according to the National Cancer Institute Common Toxicity Criteria (NCI-CTC), and Grade 1 or less for peripheral neuropathy.   (3) Even if these conditions for treatment were met, administration could be postponed at the investigator’s discretion (e.g., for a rapid decrease of the leukocyte count/platelet count, occurrence of jaundice, etc).   If any of the criteria were not met, treatment was postponed. The subsequent course could be postponed for up to 21 days (excluding the scheduled day of starting administration). If administration could not be commenced during this period, the study was discontinued. Discontinuation of therapy Administration was continued until any of the following criteria for discontinuation were fulfilled. (1) The patient was judged to have progressive disease (PD), including clinical PD.   (2) Adverse events occurred that made further administration difficult.