Maximum alpha-galactosidase yield (117 U g(-1) of dry fermented substrate) was obtained when soya bean flour supplemented with 1.5% galactose and with initial moisture content of 40% was inoculated with 1.9 x 10(6) CFU g(-1) initial dry substrate.

Conclusions: PCI-32765 nmr The model was valid and could result in considerably enhanced enzyme yield.

Significance and Impact of the Study: The results indicated a cost effective method for the production of alpha-galactosidase using soya bean flour. This is the first report on exploitation of the potential of filamentous bacterium for the production of alpha-galactosidase, an enzyme having versatile applications.”
“The

aim of this study was to determine whether striatal glial cells of adult rats with extensive nigro-striatal dopaminergic learn more denervation are induced to contain dopamine by injection of exogenous L-DOPA. At 2 weeks after injection of 6-hydroxydopamine into the medial forebrain bundle of rats, immuno-reactivity of glial cells was detected with antibodies against glial fibrillary acidic protein (GFAP) or ionized calcium binding adapter molecule 1 (Ibal) in the intact and lesioned striatum. Double-labeling immunofluorescence method was secondly performed. In the lesioned striatum, immuno-reactivity of GFAP was significantly increased, whereas immuno-reactivity

of lbal was significantly increased except for ventral portion. Exogenous L-DOPA induced DA immuno-reactivity in the striatum, which was independently detected from GFAP immuno-positive astroglial cells or lbal immuno-positive microglial cells in the intact side as well as in the lesioned side. These findings suggest that the proliferation of glial cells in the striatum is the response to the loss of dopaminergic terminals but the glial cells do not compensate for the lost dopaminergic terminals. (C) 2008 Elsevier Ireland Ltd. All rights reserved.”
“Aims: The aim of this study was to develop an approach based on a reverse transcriptase (RT)-PCR/denaturing gradient gel electrophoresis (DGGE) for the detection of the functional genes nifH and

anfH https://www.selleck.cn/products/bay80-6946.html in Paenibacillus durus.

Methods and Results: Two sets of primers were employed to study the expression of the nitrogen fixation genes in a pure-culture system of P. durus grown in media with increasing concentrations of ammonium (NH4+), tungsten (W) or molybdenum (Mo). The results obtained indicate that the expression of nitrogenase genes from P. durus can take place in the presence of relatively high levels of fixed nitrogen. It was also observed that the addition of 20 mu mol l(-1) molybdenum and 2 mmol l(-1) tungstate did not interfere in the mRNA levels of nifH and anfH genes.

Conclusions: Our results demonstrate the presence and transcription of nifH and anfH in P. durus under a variety of growth conditions.