In human cell lines AURKB similar functions in chromosome condensation, alignment and segregation and cytokinesis. We Conna Small AURKC was originally t AURKC well as a homologue in M Usen testis-specific, and in a number of human ASA404 cancer cell lines, including normal HeLa cells, where it is overexpressed AURKA localized with centrosomes identified. Lines in tissue culture cells but colocalized AURKC AURKB with centromeres and expression of Ph notyps Can AURKC multinucleation AURKB depleted store observed in cells suggesting that the function can be superimposed k Can because AURKC the AURKB. Interestingly AURKB AURKC and non-overlapping functions of the mouse have spermatogenesis. Testicular sections of M usen, The catalytically inactive AURKB contain erh Hte apoptosis in spermatocytes and meiotic w During Mice without mature sperm shape AURKC K Heads with abnormal chromatin condensation defects.
Since Aurora kinases overexpressed in many cancers, several pharmacological inhibitors have been TG100-115 developed. However, prevents the high proportion of conservation of amino Acids in the catalytic Dom NEN of the three S Uger Aurora kinases, many of these inhibitors target a kinase. ZM447439 anilino 6-methoxy-7 propoxyquinazoline inhibits recombinant AURKA and AURKB. In vitro kinase assay with IC50 values of 110 and 130 nM The two human cancer cell lines and spermatocytes with ZM447439 exposure chromosome alignment, segregation and cytokinesis errors treated. Mouse oocytes with ZM447439 treated not Met aligned progress and II contain condensed chromosomes and probably wrong hypoglycemia on the phosphorylation of histone H3 at S10 and S28.
The molecular mechanisms that understand the high incidence of aneuplo In human oocytes, we examined the state of the Aurora kinases w During meiotic maturation of mouse oocytes, where prices aneuplo The range 8% to 12%. We report for the first time the location of the three AURKs in mouse oocytes. AURKA co-localized with microtubule organizing centers, the acentriolar and polar microtubules are both Mead Mead I and II, w During AURKB focuses on the kinetochore regions of chromosomes, especially at the Met I and Met II not. W During the transition MI MII localize both AURKA and Re AURKB in the center of the spindle.
AURKC specific homologous germ cells, along the L Length of the chromosomes, including normal of the centromere region I and II Met Met According to earlier reports, dir Willingly inhibition of Aurora kinases with ZM447439 progression of meiosis and causes a displacement of chromosomes I and II Met Met is important that overexpression by AURKB in ZM447439-treated oocytes, but not AURKA or AURKC, partially reflect the alignment of chromosomes at the Met, I suggest that chromosome alignment identified M ngel k can AURKB be attributed. C. RESULTS AURKA mRNA in mouse oocytes and eggs, determine the relative H Abundance of AURKA, Aurkc Aurkb and transcripts, we isolated mRNA from mature oocytes and eggs Met II arrested sexually mature mouse. Normalization against protein kinase A mRNA, a critical regulator of meiotic resumption in the oocyte and under various anf Nglichen Taqman probes Hnlicher efficiency, we found that AURKA MRNA is the most common h At both stages compared to Aurkb and mRNA Aurkc.
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