, 1999; Nishikaku, 2003). The specificity of the immunohistochemical reaction was demonstrated by the absence of staining detected in control tissue slides without the presence

of anti-IFN-γ antibody (Fig. 1a). In omentum tissue sections of uninfected mice, only weak positivity was observed in mononuclear cells (Fig. 1b). After 15 days of Pb18 infection, IFN-γ immunostaining was detected in sparse lymphomononuclear cells at the periphery of omentum granulomas of B10.A susceptible mice (Fig. 1c). In A/J resistant mice, marked positive reaction was found in lymphomononuclear cells at the peripheral foci of necrotic lesions (Fig. 1d), which were mainly observed in this mouse strain. At 120 days post infection, B10.A mice showed disseminated loose lesions with IFN-γ stained cells circumscribing granulomatous foci (Fig. 1e). In A/J mice, MS275 intense positivity was detected in lymphomononuclear cells forming Selleckchem AZD2014 several aggregates surrounding central necrosis and compact granulomatous lesions (Fig. 1f). At this later phase of infection, the lesions developed by both mouse

strains showed marked ECM deposition, but with weak immunostaining for IFN-γ (data not shown). After 15 days of infection with the slightly virulent P. brasiliensis isolate Pb265, a similar pattern of IFN-γ staining was detected in both mouse strains when compared with Pb18 inoculated mice at the early stage of infection. Positive lymphomononuclear cells were localized at the periphery of granulomatous lesions (Fig. 2a and b). On the other hand, few IFN-γ positive cells were Decitabine found in the residual lesions of both mouse strains at the later phase of infection with Pb265 (Fig. 2c and d). Figures 3 and 4 show the quantitative analysis of IFN-γ immunohistochemical reaction. The number of immunoreactive cells was similar in the lesions of B10.A and A/J

mice after 15 days postinfection with Pb18. In contrast, the number of IFN-γ positive cells increased in both mouse strains at the later phase of infection with Pb18 (120 days), being significantly higher in A/J mice, when comparing the stage of infection (P < 0.05; 15 vs. 120 days) and also the mouse strain (P < 0.05; B10.A vs. A/J). Regarding the intensity of immunostaining at 15 days post infection with Pb18, the percentage of weakly positive cells predominated over strongly immunostained cells in the lesions of susceptible (68%) and resistant (62%) mice, whereas at 120 days post infection, the number of weakly and strongly immunostained cells was similar in B10.A (55% and 45%, respectively) and in A/J mice (50%). Many immunostained cells were found in B10.A and A/J mice at 15 days post infection with Pb265. The percentage of weakly and strongly positive cells was similar in the susceptible mice (53% and 47%, respectively), but in the resistant ones, there were higher numbers of weakly positive cells (59%).