83 100                                     64_N 35.56 35.56 100                                   64_T 39.13 43.48 66.67 100                                 1293_N 41.87 27.91 42.86 41.87 100                               1293_T 30 30 35.9 40 59.46 100                             211_N 31.11 31.11 36.37 44.45 38.1 30.77 100                           211_T 50 36.37 32.56 54.55 34.15 31.58 65.12 100                         184_T 41.87 27.91 33.33 37.21 50 32.43

42.86 58.54 100                       527_N 36.37 45.46 46.51 50 39.03 36.85 41.87 42.86 39.03 100                     527_T 42.11 31.58 32.43 42.11 34.29 31.25 43.25 44.45 45.72 50 100     BMS345541 nmr               146_N 27.27 54.55 37.21 50 34.15 21.05 32.56 47.62 48.78 52.39 44.45 100                 146_T 36.37

54.55 37.21 54.55 34.15 26.32 55.81 57.15 48.78 42.86 50 71.43 100               184_N 31.11 35.56 27.27 40 28.57 20.51 45.46 51.17 47.62 51.17 32.43 65.12 65.12 100             164_N 20.41 36.74 29.17 28.57 26.09 37.21 25 25.53 26.09 12.77 19.51 38.3 12.77 33.33 100           164_T 24.49 28.57 20.83 24.49 21.74 27.91 16.67 21.28 21.74 17.03 24.39 21.28 25.53 16.67 38.47 100         142_N 34.05 34.05 SP600125 mw 30.44 25.53 31.82 43.91 17.39 35.56 40.91 13.33 30.77 40 35.56 30.44 56.01 36.01 100       142_T 32.56 46.51 33.33 32.56 40 27.03 33.33 43.91 40 24.39 51.43 68.29 53.66 47.62 26.09 34.79 77.27 100     1457_N 43.48 21.74 22.23 21.74 41.87 30

22.23 36.37 41.87 18.19 31.58 Ribonucleotide reductase 31.82 22.73 31.11 36.74 40.82 46.81 41.87 100   1457_T 13.95 18.61 23.81 18.61 15 27.03 14.29 14.64 20 9.76 0 19.51 19.51 14.29 30.44 26.09 36.37 15 65.12 100 N–Non-tumor; T–Tumor. This GSK126 prompted us to conduct cloning and sequencing studies using 16S rDNA amplification to identify microbiotal populations at these sites. The clonal libraries with clinical distinctions were constructed with approximately 1200 high quality sequences from the rDNA inserts of non-tumor and tumor tissues. About 276 (~22.9%) sequences with <350 bases and 14 chimeric sequences (1.2%) were eliminated from analysis. The filtered 914 (75.9%) sequences of 350–900 bases from combined (non-tumor and tumor) library were characterized, of which 107 sequences (8.9%) with <98% sequence identity accounted for genus level classification and were uncharacterized at species level. The remaining 807 (67%) sequences having >98% sequence identity to 16S rRNA reference sequences in HOMD were classified to species level. Figure 3a shows the % distribution of phyla at tumor and non-tumor sites of the patient population.