930′ E144°52.351′) in northern Guam and at the Inarajan Experiment Station (N13°61.963′ E144°45.353′) in southern Guam from October 01, 2013 to January 30, 2014. Treatment plots measuring 6 × 6 m were arranged in a randomized block design and separated from other plots by 1 m buffer zones to prevent any treatment check details effect. Sweet potato cuttings of the variety IB 195 (Kuma 2) known to be highly susceptible to C. formicarius damage

( Nandawani and Tudela, 2010) were transplanted into rows 80 cm apart with 30 cm between plants within each row. Each treatment was replicated three times, for a total of 33 individual plots. Each plot consisted of 12 rows of 15 sweet potato plantings, for a total of 180 plants per plot. Fertilizer in the form of N, P, K, and S was applied at the actual time of planting according to published recommendations ( Nandawani and Tudela, 2010). Since plants require thirty days to form tubers, at which time C. formicarius infestation starts, the first treatment applications ( Table 1) were made on October 1, 2013. A pretreatment count of C. formicarius damage was taken on September 30, 2013, and subsequent counts were made on October

14, November 4 and 18, and December 02 and 16. The damage to selleck kinase inhibitor roots (tubers) in each plot was evaluated by randomly selecting eight roots from each treatment plot and counting the number of feeding holes. The yield of sweet potato as measured by tuber weight was recorded for each plot. Damage levels and yields from the treatment and control plots were compared, relative to controls, to evaluate the effectiveness of entomopathogens and low risk insecticides in reducing damage from C. formicarius. Adult weevils were collected from each plot in randomly selected 1 m2 quadrats (Reddy, 2011)

searched the surface of the ground at the same time intervals as mentioned above. Sampled insects were then incubated in the laboratory for up to two weeks and observed for mortality. Any adults failing to move when probed tuclazepam with a dissecting needle were recorded as dead and removed from the boxes. These dead adults were surface-sterilized and incubated separately in Petri dishes containing moist filter paper. The cadavers were inspected for the presence of fungal mycelium (mycosis) after 7–14 days. All mortality in each treatment was transformed to adjusted mortality (AM) according to the control (water spray). The AM was calculated as the following equation: AM=Mortalitytreatment-Mortalitycontrol1-Mortalitycontrolwhere Mortalitytreatment was the mortality of adult (C. formicarius) in each treatment while Mortalitytreatment was the mortality of adults in the control treatment. The data of AM were log-transformed to meet the normal distribution requirement, with homogeneous variance among different treatments. Then, repeated measures ANOVA was used to examine the effects of different treatments (T1: M. brunneum, T2: B. bassiana, T3: spinosad, T4: azadirachtin, T5: B. bassiana + M. brunneum, T6: B.