9899, p < 0.05). These results are similar to those in previous studies, which reported variations in the inhibitory effects of microbial pesticides against pathogen growth [9]. The degree of protection, in terms of the percentage reduction in the number of disease lesions, is displayed in Table 1. No significant difference (p < 0.05) was detected between the B. subtilis HK-CSM-1 and

ITA treatments. The TSB control also displayed a protective effect (p < 0.05) compared with the control, but lower than that of B. subtilis HK-CSM-1. Anthracnose infection processes can be divided into two stages, referred to initially as biotrophs and later switching to necrotrophs. The first biotrophic stage involves spore germination and the formation of an appressorium, then penetration into plant tissues by a thin penetration peg. In the second necrotrophic stage, the CDK inhibition invaded hypha is developed in the plant tissues, resulting in death and collapse to form a sunken area [10] and [11].

To verify the attenuation of disease symptoms, we also surveyed the differences in size of anthracnose lesions. Interestingly, as displayed in Table 1, treatment with B. subtilis HK-CSM-1 was not significantly different from the control in terms of lesion size (area). However, the disease severity was significantly reduced in plants treated with B. subtilis HK-CSM-1 compared with the controls. This suggests that B. subtilis was able to inhibit virulence at the penetration stage, but not at the tissue invasion stage. This implies that treatment during the penetration stage PD-0332991 manufacturer is critical in protecting against anthracnose. Lastly, we investigated the area of the lesions as a percentage of the total leaf area, which is equivalent to disease severity. As shown in Fig. 3 and Table 1, there was no significant difference in the control of anthracnose between B. subtilis HK-CSM-1 and ITA (p < 0.01). Furthermore, the percentage

of leaf area covered by lesions indicated significant linear correlation (r = 0.95038, p < 0.05) with the number of lesions. This again suggests that the penetration stage is critical in the effective control of anthracnose in ginseng. These observations also confirm the veracity of visual assessments. We have reported an effective approach to achieve the ecologically friendly control of ginseng anthracnose, one of the most harmful diseases of this Ponatinib solubility dmso crop. The protective effects of B. subtilis HK-CSM-1 were similar to those of the commercial fungicide ITA. However, this study was conducted on containerized plants and further studies are required to investigate whether these results hold true under field conditions. To develop an effective biological control standard, it is necessary to test the protective effects of B. subtilis in the field, including the determination of the optimum time for the treatment. In addition, formulations prolonging the survival of the bacterium on crop plants are necessary.