An important limitation both here and in previous studies is that patients with severe disease who died within the first 24–48 hours were under-represented. Around half of all deaths from melioidosis occur within the first 48 hours, and such cases are often diagnosed retrospectively once the culture results become available. This is reflected in the

overall mortality rate for the 230 study patients of 17%, which is less than half that reported from the same hospital when all cases are taken into account.5 Computerised tomography is more sensitive for detecting intra-abdominal abscesses than ultrasound and is used elsewhere to investigate patients with melioidosis, but our choice of ultrasound is based on the fact that many settings in Asia where melioidosis occurs may have access to ultrasound but not to computerised tomography. In conclusion, hepatic and splenic abscesses I-BET-762 cell line in patients with melioidosis were often multiple and clinically silent, but mortality in patients with hepatosplenic abscesses 4 weeks post-discharge was lower than in patients without abscesses. Tyrosine Kinase Inhibitor Library RRM, TV, PA, MH and GCKWK conceived the study. RRM, TV, PA, MH, PY, DL, GCKWK, WC and SJP designed the study. RRM and RJM analysed the data. RRJ, RJM, DL and NPJD interpreted the data. RRM, RJM and SJP drafted the manuscript. All authors critically revised the manuscript

for intellectual content, read and approved the final version. SJP is the guarantor of the paper. Clomifene This study was funded by the Wellcome Trust, London, UK (Grant number 087460/Z/08/Z). None. Ethical approval for this study was obtained from Sappasitthiprasong Hospital Ethical Committee (Reference number 03/2008). We thank staff at Sappasitthiprasong hospital who managed the patients enrolled in this study; Varinthorn Praikaew, Jintana Suwannapruek and Nuttapol Panachuenwongsakul

for assistance with data management; and Sukanya Pangmee and Gumphol Wongsuwan for laboratory support. “
“Orientia tsutsugamushi is an obligate intracellular bacterium and causative agent of scrub typhus. Multiplication of O. tsutsugamushi occurs in the cytoplasm of infected cells with a doubling time of between 9 and 18 h. 1 The manual enumeration of O. tsutsugamushi examined under a microscope becomes difficult when a large number of particles exist in a microscopic field. The small size of O. tsutsugamushi (0.5–2 μm) usually makes manual counting difficult as numbers of organisms increase. The ImageJ program is a Java-based open source image enumeration software package freely downloadable from the US National Institute of Health website (http://imagej.nih.gov/ij/). ImageJ has been used to enumerate malaria parasites on Giemsa-stained thick blood films and Chlamydia spp. inclusion bodies in cell culture by immunofluorescence. 2 and 3 Here we have applied ImageJ to counting of O. tsutsugamushi.