Anal Sci 2007,23(5):517–522.PubMedCrossRef 47. Molinari G, Guzman CA, Pesce A, Schito GC: Inhibition of Pseudomonas aeruginosa virulence factors by subinhibitory concentrations of azithromycin and other macrolide antibiotics. J Antimicrob Chemother 1993,31(5):681–688.PubMedCrossRef 48. Li Q, Zhou X, Nie X, Yang J: The role of recombinant human elafin in the resistance of A549 cells against Pseudomonas aeruginosa biofilm. Respiration 2010,79(1):68–75.PubMedCrossRef
49. Bourbonnais Y, Larouche C, Tremblay GM: Production of full-length human pre-elafin, an elastase specific inhibitor, from yeast requires the absence of a functional yapsin Selleckchem RG-7388 1 (Yps1p) endoprotease. Protein Expr Purif 2000,20(3):485–491.PubMedCrossRef 50. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory. New York: Cold Spring Harbor Laboratory Press; 1989. 51. Kaiser C, Michaelis S, Mitchell A: Laboratory Course Manual for Methods in Yeast Genetics, Cold BYL719 datasheet Spring Harbor Laboratory. New York: Cold Spring Harbor Laboratory Press; 1994. 52. Bourbonnais Y, Ash J, Daigle M, Thomas DY: Isolation and characterization of S. cerevisiae mutants defective in somatostatin expression: cloning and
functional role of a yeast gene encoding an aspartyl protease in precursor processing at monobasic cleavage sites. EMBO J 1993,12(1):285–294.PubMed 53. Doucet N, Savard PY, Pelletier JN, Gagne SM: NMR investigation of Tyr105 mutants in TEM-1 beta-lactamase: dynamics are correlated with function. J Biol Chem 2007,282(29):21448–21459.PubMedCrossRef 54. Doucet A, Bouchard D, Janelle MF, Bellemare A, Gagne S, Tremblay GM, Bourbonnais Y: Characterization of human pre-elafin mutants: full antipeptidase activity
is essential to preserve lung tissue integrity in experimental emphysema. Biochem J 2007,405(3):455–463.PubMedCrossRef 55. Munoz V, Serrano L: Elucidating the folding problem of helical peptides using empirical parameters. Nat Struct Biol 1994,1(6):399–409.PubMedCrossRef 56. Munoz V, Serrano L: Elucidating the folding problem of DNA ligase helical peptides using empirical parameters. III. Temperature and pH dependence. J Mol Biol 1995,245(3):297–308.PubMedCrossRef 57. Munoz V, Serrano L: Elucidating the folding problem of helical peptides using empirical parameters. II. Helix macrodipole effects and rational modification of the helical content of natural peptides. J Mol Biol 1995,245(3):275–296.PubMedCrossRef 58. Munoz V, Serrano L: Development of the multiple sequence approximation within the AGADIR model of alpha-helix formation: comparison with Zimm-Bragg and Lifson-Roig formalisms. Biopolymers 1997,41(5):495–509.PubMedCrossRef 59. Lacroix E, Viguera AR, Serrano L: Elucidating the folding problem of alpha-helices: local motifs, Alvocidib cost long-range electrostatics, ionic-strength dependence and prediction of NMR parameters. J Mol Biol 1998,284(1):173–191.PubMedCrossRef 60.