ADR-F 925, an obstacle to the UV-sensitive and selective Adrenergic Receptors detection. To date only the FPs have developed and applied analytical methods for the simultaneous analysis of DEX and its metabolites. In fact, they have very w Ssrigen mobile phases with more reactive ion-pair is used. The compositions of the mobile phase, however, are hardly compatible with MS detection, and therefore without HPLC method, which was conducted with MS detection for the simultaneous analysis of DEX and its metabolites previously published VER. Instead, the low w Ssrige / organic phase volatile portable type of analysis HILIC for MS detection rates, promises a high detector response, particularly when ESI MS. The purpose of this study systematically studywasto retention behavior of the model drug and three metabolites DEX on different station Later phases, HILIC hydrophilic compatible under the AUY922 conditions of mass spectrometry. This model is a concrete example of a stimulating analysis of a parent drug lipophilicity average together with its hydrophilic metabolites, Including Structurally related Lich positional isomers. Thus, the use of this model is clinically relevant, we have attempted to reveal the advantages and limitations of HILIC in this area of separation science. Assessed Station re-phase in this study were selected hlt To represent two large groups of e-phase for HILIC analysis of polar drugs.
The S Molecules have different chemical properties of the Procollagen C Proteinase surface Surface to determine the involvement of different retention mechanisms. Ascentis Express HILIC and Atlantis HILIC Silica-S Pillars repr sentieren Bare silica Acid. Atlantis HILIC from por This silica is complete, then express Ascentis HILIC is formed from condensed core particles. The two station Later phases of type B silica. The range of Ascentis Express HILIC pH 3.0 and 8.0, the upper temperature limit of 60. Atlantis HILIC pH of the mobile phase is limited to 1.0 5.0, and the recommended temperature range is 20 45 Silica Acid-base columns of ZIC zwitterionic HILIC and ZIC HILIC contains Obelisc N represented Lt fragment covalently to the surface Surface of the silica Sulfoalkylbetaine acid. The 1:1 ratio Ratio between strong cations and anion exchange of the results of the surface Chenladung from zero. ZIC HILIC pH range of 3.0 to 8.0, the upper temperature range 70th N Obelisc door anionic groups in the N Height of the surface Surface of the silica Acid, the cationic groups are separated in the periphery by heat Hydrophilic compounds. The Honokiol exact chemical nature of the zwitterion is not specified by the manufacturer. The stability of t Obelisc of N is limited to the pH range of 2.5 to 4.5 and temperature range of 20 to 45.
The residence time in the study with the increase of w Ssrigen fraction of the mobile phase decreases allcompoundsinvolved. The elution order is identical on all S Molecules tested. It has been found that DEX in the N Height of the void volume of all columns HILIC weight multimer Was selected, although the sw Surface of the mobile phase was used. However, ADR was relatively strong at 925 S HILIC Maintained pillars and all, so its analysis was a bit annoying. The use of the mobile phase with less than 15% of w Ssrigen Group, 925 ADR are not eluted in time in one or peak shape was not found acceptable. The h HIGHEST retention was observed on Obelisc N, where ADR was 925.