Both groups were progressed after 4 weeks of training to 70% of their predicted 1RM or

age-predicted heart rate depending on grouping. The metabolic equivalents (METs) for both the aerobic exercise and progressive resistance exercise training were estimated to be approximately 3.5 in accordance with the compendium of METs provided by the American College of Sports Medicine (ACSM 2000), a value defined as moderate intensity (Pate et al 1995). The aerobic exercise intervention is presented in Table 1. All participants wore a heart rate monitor during the warmup and exercise program and were supervised in their exercises in a group. Each participant was scheduled to complete 18 exercise sessions over 8 weeks at a frequency of 2 to 3 times a week. The primary outcome measure was HbA1c. Secondary outcomes included blood glucose, lipid profile, and anthropometric and cardiovascular measures. Alpelisib molecular weight Adverse events were also recorded. All outcome assessors were blinded to group allocation. HbA1c was measured using 10 ml of blood drawn from participants who fasted at least 10 h from the night before and analysed at the Biochemistry Laboratory of the Pathology Department in Singapore General Hospital by laboratory Panobinostat in vivo assistants who were also blinded to the project. HbA1c was measured using high performance liquid

chromatography with a coefficient of variation (CV) of 2.4% at 5.1% (HbA1c) and a CV of 1.9% at 9.6% (HbA1c). Glucose was measured using the glucose oxidase method with a CV of 1.6% at 3.3 mmol/L and a CV of 1.1% at 18.8 mmol/L. The lipid profile comprised total cholesterol, triglycerides, high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C). Total cholesterol and triglycerides were measured using enzymatic colorimetric methods with cholesterol oxidase-peroxidase amino phenazone almost phenol and glycerol-3-phosphate oxidase-peroxidase amino phenazone phenol. The CV for cholesterol is 1.9% at 3.22 mmol/L and 1.3% at

7.72 mmol/L. The CV for triglyceride is 1.8% at 1.02 mmol/L and 1.4% at 2.27 mmol/L. HDL-C was measured using homogenous enzymatic colorimetric assay with a CV of 4.8% at 0.93 mmol/L and 3.7% at 2.06 mmol/L. LDL-C was calculated using the Friedewald formula. Anthropometric measurements included weight, body mass index, body fat measured by skin fold and by bioimpedance, waist circumference and waist:hip ratio. Body mass index was calculated as weight in kg divided by the square of height in m. Skin-fold thickness was measured at four sites: biceps, triceps, sub-scapular, and suprailiac, on the right side of the body (Heyward 2002), and percentage body fat was estimated using a formula applicable to Singaporeans (Deurenberg-Yap et al 2003). Percentage body fat was also measured using two-point bioimpedance analysisa and a regression equation based on measured resistance and reactance.