From the principle of lowest thera peutic dose with longest time window, the optimized composition have been A3B2, that may be to say the ideal therapeutic dose and time window was ischemia 2. 0 h with picroside II ten mg kg body fat by intraperitoneally injection. Gray value of myelin, There was a significant difference among the unique levels of impact elements A within the expression of myelin, though no major probability discovered amongst affect factor B and element C. This indicated the therapeutic time window had drastically influence within the ex pression of myelin after cerebral ischemia damage, though no sizeable influence existed in different drug doses and time dose interactions. LSD showed that unique administration time concerning one. 0 h and 1. five h, one. 0 h and 2. 0 h, one. five h and two. 0 h, 1.

five h and 2. 5 h, 2. 0 h and two. 5 h had significant distinctions, no significant dif ferences identified between the rest groups. There was no statistical significance concerning groups on dose. So, the ideal blend is A3B2, that’s injecting picroside II 10 mg kg entire body weight at cerebral ischemia two. 0 Avagacestat molecular weight h. Ultrastructure of myelin by TEM, In control group, the framework of myelin sheath was distinct and neat, and the neural axon was in focus, while unclear and irregular as well as neural axon disappeared in model group, as well as damaged myelin sheath in treatment group recovered sig nificantly evaluating with model group. Relative content material of protein by WB, Quantitative detection in the expression of MBP showed that vary ent intensity of MBP protein expressed in numerous groups, and it had been significantly higher than that in model group right after remedy.

selleck chemical Roscovitine Examination of variance showed that the unique ranges of issue A had sig nificant differences within the expression of MBP, but no major distinctions in issue B and aspect C. LSD indicated that substantial deviations were discovered in between 1. 0 h and one. 5 h, one. 0 h and 2. 0 h, one. 5 h and 2. 5 h, 2. 0 h and two. 5 h in therapeutic time window, but no major deviations among the rest therapeutic time ranges. The differ ent therapeutic dose ranges had no considerable differ ences. Synthetically, the most effective mixture is A3B2, i. e. the very best therapeutic time window and dose of picroside II ought to be injecting intraperitoneally with 10 mg kg entire body excess weight at cere bral ischemia two. 0 h. Relative abundance of mRNA by RT PCR, The expressions of MBP mRNA differed between numerous groups, and greater markedly than that in model group following treatment method. The results of ANOVA showed there was statistically big difference in different levels of factor A on MBP mRNA, and no important distinction uncovered in aspect B and issue C.