melanogaster (hsp70-lacZ) Bg9. CONCLUSION 1-methyl-2-phenylindole method for the estimation of lipid peroxidation was found to be precise, accurate, and linear. Satisfactory results were obtained from this method. The most interesting feature of this method is its specificity for the measurement of MDA. The method is recommended for the estimation of lipid peroxidation in the third instar larvae of transgenic D. melanogaster (hsp70-lacZ) Bg9. Footnotes Source of Support: Nil Conflict of Interest: None declared.
Olmesartan Medoxomil (OLME) is chemically (5-methyl-2-oxo-2H-1,3-dioxol-4-yl)methyl 4-(2-hydroxy propan-2-yl)-2-propyl-1-(4-[2-(2H-1,2,3,4-tetrazol-5-phenyl]phenylmethyl) -1H-imidazole-5-carboxylate [Figure 1]. OLME belongs to a class of drugs known as angiotensin II (A2) receptor blockers (ARBs). These medicines are closely related to the common medications known as ACE inhibitors, which block an enzyme in the body that is responsible for causing the blood vessels to narrow.[1,2] Metoprolol succinate (METO) is chemically (RS)-1-(Isopropylamino)-3-[4-(2- methoxyethyl)phenoxy] propan-2-ol succinate [Figure 2], is a cardio selective ��-blocker, used in the treatment of hypertension, angina pectoris, arrhythmia, myocardial infraction and heart failure. It is official in Indian Pharmacopoeia (IP) and British Pharmacopoeia (BP). IP and BP describe potentiometric method for its estimation.[3,4] Literature review revealed that few methods have been reported for the OLME which includes, LC-DAD method for its determination in tablets exposed to stress conditions,[5,6] RP-HPLC method for determination of the OLME in combination with hydrochlorotiazide[7] and amlodipine.[8] A few methods have also been reported for METO which includes RP-HPLC method for its determination in combination with ramipril[9] and amlodipine.[10] The stability of a drug substance or drug product is defined as its capacity to remain within established specifications, i.e., to maintain its identity, strength, quality, and purity until the retest or expiry date. Stability testing of an active substance or finished product provides evidence of how the quality of a drug substance or drug product varies with time under a variety of environmental conditions, for example temperature, humidity, and light. Knowledge from stability studies is used in the development of manufacturing processes, selection of proper packaging and storage conditions, and determination of product shelf-life.[11,12] With the aim to develop accurate, precise and selective reverse phase isocratic HPLC assay procedure for the analysis of the titled analytes in individual bulk drug samples and in combined dosage formulation, the study was undertaken. Various trials on conventional C8 and C18 columns were conducted using different buffers including sodium phosphate, potassium phosphate in the wide pH range of 3 to 10.