ws so significnty reduced see, M. dditiony, the ceur ociztion of PDGFR downstrem basic research meditors ws determined by doube immu nofuorescence stining. Simir to the PDGFR , MMP , MMP,phosphorp immunorectivity were miny fou in the neurovscur structure,  Neohesperidin incuding strocytesthe eothei ces,MMP ws ony fou in the eothei ces Suppement. PDGFR ctivtion Incresed Brin Edem PostbICH thours postPDGF deivery, neurobehvior deficits were evuted using the modified Grci test Suppement corner turn test Suppemen tB. Our resuts reveed no difference in deficit severity pred to vehice tretment nims, though out ofnims with PDGF injection died inhours. We so fou tht the brin edem in the ipsit er bs gngi ws significnty incresed pred to vehice nims ipsiBG:

PDGF,vs vehice SuppementChours fter PDGF deivery. PDGFR ctivtion Impired BBB Integrity But Ws Reversed Using p MPK Inhibitor in Nı¨ve Mice thours foowing PDGF Mycophenolate mofetil injection, Evns bue extrvstion ws significnty incresed in the ipsiter hemisphere pred to mice injected ony with PBS BBB permebiity ws so detected hour foowing PDGF injection. The resuts showed tht the Evns bue extrvstion ws so incresed pred to PBSony injection  . p MPK inhibitor,hydrochoride, ws coinjected with December PDGF into the right bs gngi of n ¨ ı ve mice. Twentyfour hours ter, we fou tht the Evns bue extrvstion ws significnty diminished pred to nims injected with PDGF seeB. ombin Inhibition Preserved BBB Integrity, Whie Suppressing PDGFR ctivtionPDGF Expression PostbICH The ombin inhibitor, hirudin, ws coinjected with utoogous rteri bood into the right bs gngi of mice. Twentyfour hours foowing hirudin injection, Evns bue extrvstion ws significnty reduced in hirudininjected nims pred to vehice nims Hirudin tretment so significnty improved neuroogic scores foowing the modified Grci test Suppement , but fied to show improvement with the corner turn test Suppe ment B.

Our resuts demonstrted tht the eve of phosphoryted PDGFR seeB, CPDGF seeD, E were both significnty decresed in hirudintreted nims pred to vehice nims  .hours postbICH. PDGFR ctivtion Reversed the Protective Effects of ombin Inhibition on BBB Integrity PostbICH Our resuts demonstrted tht Evns bue extrvstion ws significnty incresed pred to mice treted ony with hirudin  .hours foowing hirudinPDGF coinjection. The protection buy Taxifolin sserted by hirudin on neurobehvior function ws reversed fwing PDGF dministrtion in the modified Grci test Suppement but not in the cor ner turn test SuppementBhours fter injec tion. dditiony, we sbserved tht the eve of phosphorytion of PDGFR significnty incresed by PDGF pred to mice treted ony with hirudin  .hours fter injection seeB, C. PDGFR Suppression Reduced ombinIuced BBB Impirment ough the PDGFR pMMPs Pthwy Our resuts showed tht Geevec tretment significnty diminished Evns bue extrvstion pred tm bininjected   .

Phosphoryted PDGFR ws significnty incresedhours foowing ombin injectionsignificnty reduced in the Gee vectreted mice pred to mice injected ony with ombin seeB, C. Geevec tretment significnty reduced the MMP  . but not MMP SuppementChours foowing ombin purchase Taxifolin injection. Simiry, MMP SuppementD, EMMP SuppementF, G NNS of Neuroogy URE : Chrcteriztion of PDGFR pthwy thours foowing bICH in mice. PDGFR ntgonist, Geevec mgkg ws dministered hour foowing bICH. Immunoprecipittion ssy IP for phosphorPDGFR eve with phosphotyrosine specific ntibody Ptyr in the ipsiter hemisphere in sh vehice,G tretment mgkg mice. The precipitted pro tein ws so visuized with PDGFR specific ntibodies Rph. Immunogobuin G IgG ws visuized s oding contro. C Getin zymogrphy ssy for MMPMMP ctivity in the ipsiter hemisphere in sh vehice,G tretment mgkg mice; Western bot ssy for F MMP, H MMP, J JNKpJNK, ErkpErk, ppp, pTF in the ipsiter hemisphere in sh vehice,G tretment mgkg mice.