On the other hand, vit E (low dose) and Se (Low dose) elicited slight significant decrease in GPx activity

as compared to normal control group. Meanwhile, MSG (high dose) followed by administration of either vit E (high or low dose) or followed by Se at high or low dose elicited slight decrease in GPx activity with respect to normal control group. At the end of the 30 days of treatment, the spermatogenic cells in the seminiferous tubules appeared to have normal histological structure in control group. Testis of the male control treated rats appears to be oval in shape and showed normal seminiferous tubules – surrounded by few edematous stroma containing NVP-BEZ235 small groups of leydig cells (Fig. 1A). In the MSG-treated group with high dose, seminiferous tubules were observed

filled by spermatogenic cells with few sperm formation and showing seminiferous tubules filled by spermatogonia only with few sperm formation (Fig. 1B and1 C). Meanwhile, in group 3 (vit E treated group PLX4032 in vivo high dose) there were testicular tissues showed normal seminiferous tubules surrounded by small groups of leydig cells (Fig. 1D). On the other hand, in group 4 (Se-treated group high dose), testicular tissues showed seminiferous tubules lined by layers of spermatogenic cells up to sperm formation (Fig. 1E). While, group 5 treated with (MSG + vit E at high dose) showed seminiferous tubules lined by few layers of spermetrogenic cells and few sperms (Fig. 1F). DNA Damage inhibitor While group 6 treated with (MSG + Se at high dose) showed seminiferous tubules lined by few layers of spermetrogenic cells and moderate number sperms (Fig. 1G). Fig. 2, Fig. 3, Fig. 4 and Fig. 5 Lipid peroxidation is one of the main processes of oxidative damage, which plays a critical role in the toxicity of many xenobiotic (Ognjanović et al., 2010). It was evaluated by assessment of TBARS [36]. In the present study, TBARS levels also

increased in the MSG treated rats. It is known that MSG produces reactive oxygen species. Therefore, antioxidant enzymes could play a crucial role on MSG toxicity [37]. Our results was in harmony with Tezcan et al. [38] who declared that MDA is one of the final decomposition of lipid peroxidation and it is also formed as a product of the cyclooxygenase reaction in prostaglandin metabolism and this assure our finding who conclude the presence of oxidative stress in rats treated with MSG in which there was high level of MDA. In agreement with previous study, the susceptibility of spermatozoa to oxidative stress as a consequence of the abundance of unsaturated fatty acids in the sperm plasma membrane and a very low concentration of cytoplasmic antioxidants is well known [39]. We demonstrated that the major reason for damage of testicular tissues is the increasing level of lipid peroxidation and these findings was in parallel with Aitken et al.