ove the security of TNP 470 before and after administration, and the microspheres were prepared successfully. This study seeks to improve the balance and the ability to provide a sustained release of the preparation of micro spheres which permit a better release length of the active drug. TNP 470, poly D,L lactic order Decitabine acid of a mean molecular weight of 11 000 was used as a company. A medium-chain triglyceride was used as an additive. Poly vinyl alcohol around 2200 levels of polymerization was used as a prime type solvent. Dichloromethane and the other reagents were of high purity level. TNP DDS was organized with a solvent evaporation method emulsion method.. The composition ratio is shown in Table 1. TNP 470 was dissolved in MCTG and PLA was included with this solution. DCM was subsequently included, solubilizing this mixture. This DCM s-olution was put into 0. Five hundred v/v PVA aqueous s-olution at 1-5 8C and stirred with a appliance to produce a W/O emulsion. The emulsion was stirred for 2 h to escape DCM and caking of TNPDDS. The TNP DDS was recovered by centrifugal Eumycetoma separation, filtered and dried in a vacuum. The control microspheres were made by exactly the same approach but with the exclusion of MCTG. Products were prepared with different composition ratios as given in Table 1. The particle shape was observed under a scanning electron microscope. The particle diameter was measured with image analysis equipment, and the distribution of the typical particle diameter and particle diameter were obtained by these effects. Cross-sections of products E and H were observed underneath the SEM. Twenty milligrams of the TNP DDS was dissolved in 1 ml of acetone and stirred after the addition of 10 ml of physiological saline. The precipitate was removed using a membrane filter. The same volume of acetonitrile was added to offer the s-olution and then stirred. The focus of TNP 470 in the s-olution was measured by high performance liquid chromatography, which contained a 490E plan numerous wavelength detector and a 510 type pump. The order was a Nucleosil 5 C18 4:6 250 mm2. The measurement was performed employing a mobile phase of 50% v/v acetonitrile solution. The flow rate was 1. The detection wavelength and 0 ml/min was 217 nm. One milligram of TNP 470 was dissolved in 5 ml of physiological saline at 37 8C. The physiological saline was occasionally tested. Each time, acetonitrile of-the same volume was added and the TNP 470 attention in the solution was measured by HPLC. The half-life of TNP 470 was calculated and the decay constant calculated from these results. TNP DDS was periodically recovered by centrifugation at 5000 rpm for 5 min. The amount of TNP 470 in the answer and the TNP DDS was tested. summarizes the properties of TNP DDSs prepared with different composi