AdipoR1 Antique Body Recogn t Analysis of variance revealed significant effects of treatment, time and 37 kDa protein (Fig. 1A), corresponding to the time AdipoR1 and PDGFR Inhibitors Adi-treatment. Post-hoc analysis of receptor proteins POR2 respectively. Immunocytochemistry showed that globular Increased adiponectin re Ht fa Typical cell is F Staining with antibodies Best against AdipoR1 and AdipoR2 rpern Expression of AdipoR1 and AdipoR2 saturated in some proliferation at 48 h (p ment (Fig. 2 D). 5) and 72 h (p 05) Post-Processing-30. December 2011 VOLUME 286 No. 52 44 915 Journal of Biological Chemistry by jbc at NYU School of Medicine Library, 6 M Downloaded March, 2012 Page 3 – FIGURE 3 neurogenesis and adiponectin. Effect of adiponectin on the cleavage of PARP in adult hippocampal neurons cultured cells shore hip stem precursor. Adult neural stem cells hippocaampal shore Preferences were With different doses of globular Re adiponectin (GAD) were incubated for 48 h. Cleavage of the precursor Shore-determined PARP protein by Western blot analysis-ERN.
A immunoblot, representative of the entire L Length and cleaved PARP. B, cleaved sumatriptan quantitative data showing the effect of adiponectin on PARP. C, quantitative data showing the effect of adiponectin to the full L Length PARP. Data are presented as mean SE (error bars), No. 4 per group. Effect of adiponectin on apoptosis of neurons in the hippocampus of adult stem cells precursor Shore cells A diponectin was reported to induce apoptosis in various cancer cell lines (45). Thus, we investigated whether adiponectin affects apoptosis in hNSCs. Using the test PARP cleavage, we have shown that the treatment had agreements with various doses of globular adiponectin for 48 h FIGURE 4. Effect of adiponectin on the differentiation of adult neural precursor Shore cells of the hippocampus cell stempro A, the cells were spherical with Shaped adiponec tin (Gad, 3 g ml) incubated with 1 MS Acid retino And containing % FBS in culture medium for 6 days.
The differentiation into neuronal or glial cell line had no significant effect on cleaved PARP (rated R (3,8) 0.238, p by examining a neuronal marker Tuj1 or GFAP, an astrocyte marker. 0.868) (Fig. 3) or full length length PARP levels (F (3,8) 86, 0.966 p) (Fig. 3). These data indicate that apoptosis of hNSCs not by adiponectin treatment is not influenced. To determine the influence of adiponectin on the differentiation of adult hippocampus-pal neural stem cell precursor Bank cells o the effect of adiponectin on the differentiation of skeletal muscle hNSCs in the neuronal or glial line, the cells were treated with 3 g ml globular re adiponectin in differentiation medium with 1 MS acid retino and that % FBS for 6 days. Double-labeling immunocytochemical F Staining was performed with anti-Tuj1, to assess the cell differentiation in neural line and with anti-GFAP to assess the differentiation of glial cells in the line. The S Acid retino That induces the differentiation of the data as mean SE (error bars), n 3 per group shown. B, represented TIVE-images showing immunocytochemical F Staining for Tuj1 and GFAP, and contrast.
DAPI nuclear scale bar, 4 . Effect of AMPK inhibition on p38MAPK and adiponectin-induced cell proliferation in adult hippocampus neural precursor Bank cells StemPro o determine whether the activation of AMPK signaling pathways mediated by adiponectin-induced proliferation of hNSCs cells were incubated with different doses of the compound inhibitor of AMPK C ( 2 M) for 2 h before incubation for 48 h pretreated to 3 g ml adiponectin globular clusters. Ing cell proliferation was measured using the MTT assay. ANOVA HNSCs either in neurons (p 63) or glial cells (p 8), no significant treatment effect (F (7.43) 7.820 showed significant p affected by the treatment of globular adiponectin (4). In addition, had adiponectin treatment had no effect on the spontaneous differentiation hNSCs.