After Hxb administration, sleep latency and total sleeping time were recorded as parameters of sedative activity. Assessment of anticonvulsant activity against PTZinduced seizures. To evaluate the presence of anticonvulsant activity, a PTZ induced seizure test was performed. PTZ at a dose of 80mg/kg was administered to the mice to induce seizures. Time to the Masitinib appearance of first seizure, percentage inhibition of convulsions and percentage inhibition of death were recorded. Mice were observed over a period of 30min. The absence of an episode of clonic spasm of at least 5 s duration indicated the ability of the extract or drug to abolish theeffect of PTZ on seizure threshold, and these animals were accepted as being protected from seizures. Statistical analyses. GraphPad Prism 3.
0 software was used for statistical analyses of the experimental data. Significant differences between Irbesartan Avapro groups were determined by one way analysis of variance followed by Tukey’s test. The results were expressed asmean standard error of the mean. Differences between datasets were considered as significant when the p value was less than 0.05.The results from the hole board tests are shown in Fig. 1. Dzm at a dose of 2 mg/kg and HME at all administered doses caused a statistically significant decrease in the total number of head dips. Pre treatment with Flu antagonized not only the effects of Dzm but also the effects of 100mg/kg HME. Nlx and Atr pre treatment did not cause any significant changes in the total number of head dips induced by the 100 mg/kg extract.
In the activity cage tests, administration of 50 and 100 mg/kg doses of HME caused significant decreases in the number of horizontal movements compared with the values observed in the control animals. The HME was also effective in decreasing the number of vertical movements in activity cage measurements at the 25 mg/kg as well as 50 Ramelteon 196597-26-9 and 100 mg/kg doses. As expected, Dzm at 2 mg/kg induced a significant decrease in the spontaneous locomotor activities of mice in both directions. Pre treatment with Flu antagonized the effects of Dzm and 100 mg/kg HME in activity cage tests, whereas Nlx and Atr did not cause any changes.In the Hxb induced sleeping tests, Dzm at 2 mg/kg and HME at 50 and 100 mg/kg doses induced significant reductions in sleep latency and significantly prolonged the sleeping time of the mice.
Pre treatment with Flu antagonised the effects of Dzm and 100 mg/kg HME, whereas Nlx and Atr did not cause any change in the effects of the 100 mg/kg extract. Table 1 indicates that Dzm at 2 mg/kg and HME at 50 and 100 mg/kg doses significantly and dosedependently delayed the onset of PTZ induced tonic convulsions, protected the GW786034 635702-64-6 animals from convulsions, and prevented PTZ induced death, compared with the control group. Pre treatment with Flu antagonized the anticonvulsant effects of Dzm and 100mg/kg HME in the convulsion tests.In this study, the putative reasoning effects of HME on the CNS of mice were investigated using different behavioural methods. Hole board and activity cage tests were used for assessing exploratory behaviour and spontaneous locomotor activity, respectively. The possible sedative effect of the extract was investigated using the Hxb induced sleeping test, and the potential anticonvulsant effect .