Rats underwent fear conditioning on Day 1. On Day 2, they underwent extinction training during the metestrus phase of the cycle (low estrogen and progesterone). Extinction recall was assessed on Day 3. Systemic injections of estrogen receptor-beta and -alpha agonists and of estradiol were administered at different time points to assess their influence on extinction consolidation and c-Fos expression in the vmPFC and amygdala. In parallel, healthy naturally cycling women underwent an analogous fear conditioning extinction training in a 3T functional magnetic resonance scanner. Measurement of their estradiol levels and skin conductance responses were obtained throughout the experiment.\n\nResults:

In female rats, administration of the estrogen-receptor beta (but not alpha) agonist facilitated extinction recall. Immediate (but not delayed) postextinction training GS-7977 price administration of estradiol facilitated extinction memory consolidation and increased c-Fos expression

in the vmPFC while reducing it in the amygdala. In parallel, natural variance in estradiol in premenopausal cycling women modulated vmPFC and amygdala reactivity and facilitated extinction recall.\n\nConclusions: We provide translational evidence that demonstrates the influence of endogenous and exogenous estradiol on the fear extinction network. Our data suggest that women’s endogenous hormonal status should be considered in future neurobiological research related to anxiety and mood disorders.”
“Dendritic cells (DCs) behave Selleckchem SRT1720 as antigenic or tolerogenic immune response inducers depending on the nature Selleck PF-562271 of their precursors, their differentiation pathway and their environment. As professional antigen presenting cells (APCs) it has been tempting to genetically modify them in order to improve their capacity to mount appropriate protective immune responses. Gene transfer may also be helpful to investigate fundamental issues about the DC biology. Of note, almost all strategies to deliver genes or interfering RNA into DCs have

been used with different success rates. These methods are non-exhaustively presented and discussed here. We focused our attention on promising in vitro as well as in vivo lentiviral-mediated gene delivery solutions into murine or human DCs.”
“Background. Mitral valve (MV) repair for posterior mitral leaflet (PML) prolapse has proven excellent results. The loop technique, which involves insertion of polytetrafluoroethylene neochordae while preserving the native PML tissue, was developed to facilitate MV repair through a minimally invasive approach. The aim of this study was to assess the medium-term results of the loop technique in comparison with the widely adopted leaflet resection technique for repair of isolated PML prolapse.\n\nMethods. Between March 1999 and January 2008, a total of 1,708 patients underwent minimally invasive MV repair. Six hundred and seventy patients (39.