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“Sequence characterized amplified region (SCAR) markers were developed to selectively detect Korean hwanggi medicine (Radix Astragali) from imported ones from China. The SCAR markers were designed based on the nucleotide sequence of the psbA/trnH intergenic region (cpDNA) and significant bands obtained by inter-simple sequence repeat (ISSR) analysis. Nutlin-3 in vitro The psF/trnH marker was developed was based on the inserted sequence (TAAAA) within all of the Chinese
samples (imports and local). Based on UBC 850 primer-amplified specific bands from all Chinese hwanggi medicine, the 15F/15R marker was designed. This primer showed high stability through 6 repeated amplifications, whereas psF/trnH marker showed 50% stability. Therefore, the designed SCAR marker (15F/ 15R) could be able to identify Korean hwanggi medicine as well as that imported from China.”
“Background: The aim of this study was to investigate the relationships between plasma visfatin, insulin resistance, lipid profile and anthropometric measurements in obese children.
Subjects and methods:
Plasma levels of visfatin, insulin, glucose, lipid profile and anthropometric indices were determined in 30 obese children and compared with those in 30 age- and gender-matched non-obese children. Visfatin was MK-2206 in vivo measured with enzyme-linked immunosorbent assay and logarithmically transformed to log visfatin for parametric comparisons.
Results: The obese group had significantly elevated plasma visfatin, fasting glucose and insulin and homeostasis model assessment (HOMA) values, as well as elevated lipid concentrations, compared
with non-obese children. In the obese group log visfatin correlated positively with weight (p = 0.007), waist circumference click here (p = 0.007), hip circumference (p = 0.034), BMI (p = 0.005), insulin (p = 0.041) and HOMA (p = 0.044). No correlation was found between visfatin and lipid profile in obese children (p >0.05). Linear regression analysis revealed significant positive relationships between log visfatin and BMI (p = 0.005), insulin and BMI (p <0.001), and between HOMA and BMI (p <0.001) in the obese group but not in the control group. Multivariate regression analysis with log visfatin as a dependent variable showed that only BMI (p = 0.005) and body weight (p = 0.014) correlated positively with log visfatin in obese children.
Conclusions: An increased visfatin concentration may be associated with BMI and insulin resistance In obese children. Although these findings may lay a foundation for further hypotheses, the limited sample size in the present study means that longitudinal studies with more patients are needed.