Streptococcus suis is an important pathogen associated with a wide range of diseases in pigs, including meningitis, septicaemia, pneumonia, endocarditis and arthritis (Staats et al., 1997; Gottschalk & Segura, 2000). Thirty-three serotypes (types 1–31, 33 and

1/2) have been described based on capsular polysaccharides, and S. suis serotype 2 (SS2) is most commonly associated with diseases in pig, and is the most frequently reported serotype worldwide (Higgins et al., 1995; Hill et al., 2005). Streptococcus suis is also the causative agent of serious infections in humans, especially in those in close contact with swine or pork byproducts (Gottschalk et al., 2007). Cases of S. suis infection have been sporadically reported from Thailand (Rusmeechan & Sribusara, 2008; Wangsomboonsiri et al., 2008), the United Kingdom (Watkins et al., 2001), Portugal (Taipa et al., 2008), Australia (Tramontana et al., 2008), the Netherlands (van Ku-0059436 concentration de Beek et al., 2008) and the United States (Smith et al., 2008; Fittipaldi et al., ALK inhibitor 2009). However, the mechanisms of its pathogenesis and virulence are not completely understood (Gottschalk & Segura, 2000), and attempts to control the infection are hampered by the lack of an effective vaccine. Several approaches have been adopted to develop effective vaccines for S. suis, but little success was achieved because the protection was either serotype- or strain dependent. In some instances,

the results were ambiguous when using killed whole cells or live avirulent vaccines (Pallares et al., 2004), and they also had the disadvantage that the presence Sulfite dehydrogenase of some components in whole-cell vaccine probably induces a dominant but nonprotective response and sometimes causes serious side effects (Liu et al., 2009). More recently, interest has shifted towards protein antigens of S. suis as vaccine candidates. Subunit vaccines using suilysin (Jacobs et al., 1996) or muramidase-released protein and extracellular protein factor (Wisselink et al., 2001) have been shown to protect pigs from homologous and heterologous SS2 strains, but in some geographical regions their application

is hindered by a substantial number of virulent strains that do not express these proteins. Although some proteins had been identified as vaccine candidate antigens (Okwumabua & Chinnapapakkagari, 2005; Li et al., 2006, 2007; Feng et al., 2009; Zhang et al., 2009a, b), identifying additional novel protective antigens is necessary to develop vaccines for pigs against S. suis. For many bacteria, the outer surface structures are attractive candidate antigens for vaccines. Many surface immunogenic proteins have been identified by immunoproteomics (Geng et al., 2008; Zhang et al., 2008). Among these, SSU05_0272 (hypothetical protein 0272; HP0272), which was annotated as ‘translation initiation factor 2’, was attractive but showed low sequence homology.