uclear division continues until 14 dai. Cell cycle activation in giant cells has also been observed by Engler et al. In that study, the transi tion from S to G2 and G2 to M phase was reported after the over expression SKLB1002 of a GUS gene driven by the cycB2 or cycA2 promoters at one to nine days after infection with M. Inhibitors,Modulators,Libraries incognita. Expression of the CDKB2 gene at 12 dai was higher than at 10 wai, i. e. 5. 2 versus 3. 1 fold, respectively. Ramsay et al. found that cyc D3 is essential to stimulate the G1 phase of the cell cycle in root knot nematode infected giant cells. In this investigation, the two types of CycD3 were shown Inhibitors,Modulators,Libraries to be relatively more strongly expressed as compared to that of LeCycA1. 1, LeCycB1. 1 and LeCycD3. 1 in giant cells induced by Meloidogyne spp. compared with other cyclin dependent kinases.

They observed PCR amplification of CyD3. 2 and Inhibitors,Modulators,Libraries CycD3. 3, while no amplification of cycA. 1, CycB1. 1 and CycD3. 1 was observed. Our data showed a suppression of gene expression of the gene encoding cycD3 which is important for the regulation of the G1 S transition. In addition, at 10 wai we found an increase in gene expres sion of CKS1, a protein that prevents CDK from driving the cell cycle into S phase. This result suggests that at the earlier time point, the giant cells reach maturity and then the genes required for nuclear division are turned off. Cell wall modification and remodeling Due to multiple nuclear divisions of selected cells with no coincident cell division, the giant cells sometimes reach more than 400 times the size of a normal cell and may contain more than one hundred nuclei.

olved in cell wall extension and remodeling. Inhibitors,Modulators,Libraries We found that the genes AV-951 encoding a cell wall modifying xyloglucan endotransglycosylase hydrolase and endoxyloglucan transferase A2 are dif ferentially expressed in soybean roots after infection with M. incognita. These enzymes play a role in softening and breaking down the cell wall. Genes encoding many endo 1,4 glucanase family members were up regulated at both time points. Endo 1,4 glucanase is involved in cell wall remodeling and expansion. LCM was used to isolate giant cells formed in tomato by M. javanica to examine gene expression. Numerous transcripts of genes involved in cell wall remodeling were also identified in the cDNA library of giant cells 4 dai, including transcripts of genes encoding pectin methy lesterase and pectinesterase.

Goellner et al. identi fied genes encoding endo 1,4 glucanases that were up regulated in feeding cells formed by M. incognita Imatinib Mesylate structure and cyst nematode in tobacco plants. Also, Mitchum et al. found that the promoter of an endo 1,4 b gluca nase gene was strongly activated in feeding cells formed by Meloidogyne incognita as indicated by strong promoter driven GUS expression. The increase in expression of the gene encoding expansin A in our results is consistent with other inves tigations, wherein the expansin genes in A. thaliana and tomato were shown to be up regulated in developin