9% and 99.8% sequence identity, respectively. Figure 1 Images of Rhizobium leguminosarum Enzalutamide molecular weight bv. trifolii strain TA1 using scanning (Left) and transmission (Center) electron microscopy as well as light microscopy to visualize colony morphology on solid media (Right). Table 1 Classification and general features of Rhizobium leguminosarum bv. trifolii strain TA1 according to the MIGS recommendations [14]. Figure 2 Phylogenetic tree showing the relationship of Rhizobium leguminosarum bv. trifolii strain TA1 (shown in blue print) with some of the root nodule bacteria in the order Rhizobiales based on aligned sequences of the 16S rRNA gene (1,307 bp internal region). … Symbiotaxonomy Rhizobium leguminosarum bv. trifolii strain TA1 is currently the commercial inoculant for white (Trifolium repens), red (Trifolium pratense) and strawberry (Trifolium fragiferum) clovers in Australia.

TA1 in general is not as effective for nitrogen fixation on annual clovers as other strains, such as WSM1325 [34,35]. However TA1 is of particular interest because it displays a broad host range for nodulation and nitrogen fixation across annual and perennial clovers originating from the European and Mediterranean centre of origin of clovers [1]. TA1 is generally able to nodulate but unable to fix with many annual and and perennial clovers originating from Africa and America [34]. Genome sequencing and annotation information Genome project history This organism was selected for sequencing on the basis of its environmental and agricultural relevance to issues in global carbon cycling, alternative energy production, and biogeochemical importance, and is part of the Community Sequencing Program at the U.

S. Department of Energy, Joint Genome Institute (JGI) for projects of relevance to agency missions. The genome project is deposited in the Genomes OnLine Database [33] and an improved-high-quality-draft genome sequence in IMG. Sequencing, finishing and annotation were performed by the JGI. A summary of the project information is shown in Table 2. Table 2 Genome sequencing project information for Rhizobium leguminosarum bv. trifolii strain TA1. Growth conditions and DNA isolation Rhizobium leguminosarum bv. trifolii strain TA1 was grown to mid logarithmic phase in TY rich media [36] on a gyratory shaker at 28��C. DNA was isolated from 60 ml of cells using a CTAB (Cetyl trimethyl ammonium bromide) bacterial genomic DNA isolation method [37].

Genome sequencing and assembly The genome of Rhizobium leguminosarum bv. trifolii strain TA1 was sequenced at the Joint Genome Institute (JGI) using a combination of Illumina [38] and 454 technologies [39]. An Illumina GAii shotgun library which generated 66,421,308 reads totaling 5,048 Mb, and a paired end 454 library with an average insert size of 13 kb which generated 393,147 reads Entinostat totaling 100.1 Mb of 454 data were generated for this genome.