94E-31 128 0045944: positive regulation of transcription from RNA polymerase II promoter 2.21E-18
73 0045893: positive regulation of transcription, DNA-dependent 7.64E-14 89 0007275: multicellular organismal development Stattic mw 1.99E-13 57 0007165: signal transduction 1.16E-10 69 0007399: nervous system development 8.52E-10 74 0006915: apoptotic process 1.76E-09 57 0045892: negative regulation of transcription, DNA-dependent 4.03E-09 55 0007155: cell adhesion 5.06E-08 90 0007411: axon guidance 9.83E-08 24 KEGG Pathways Pathway Hyp* Genes 05200: Pathways in cancer 1.84E-05 33 04010: MAPK signalling pathway 3.62E-05 31 04144: Endocytosis 1.89E-04 19 04510: Focal adhesion 2.34E-04 25 04810: Regulation
of actin cytoskeleton 4.11E-04 22 04350: TGF-beta signalling pathway 8.67E-04 12 04141: Protein processing in endoplasmic reticulum 2.19E-03 18 04630: Jak-STAT signalling SHP099 research buy pathway 5.07E-03 15 04310: Wnt signalling pathway 5.29E-03 14 04520: Adherens junction 5.68E-03 10 Panther pathways Pathway Hyp* Genes P00057: Wnt signalling pathway 6.66E-09 36 P00012: Cadherin signalling pathway 8.93E-06 20 P00018: EGF receptor signalling pathway 1.25E-04 18 P00034: Integrin signalling pathway 4.11E-04 17 P00021: FGF signalling pathway 8.83E-04 14 Abemaciclib research buy P00047: PDGF signalling pathway 2.18E-03 13 P00060: Ubiquitin proteasome pathway 2.67E-03 11 P00048: PI3 kinase pathway 5.06E-03 8 P00036: Interleukin signalling pathway 6.23E-03 11 P04393: Ras pathway 7.82E-03 10 The number of predicted target genes in the process or pathway is shown. Experimental validation of the expression levels of the most deregulated miRNAs in patients with PDAC To determine if the ten most deregulated miRNAs from the meta-analysis
(miR-155, miR-100, miR-21, miR-221, miR-31, miR-143, miR-23a, miR-217, miR-148a and miR-375) could be used as diagnostic biomarkers of PDAC, the expression levels of these miRNAs were compared between PDAC tissues and neighbouring noncancerous tissues by qRT-PCR analysis. The results showed that the expression levels of miR-155, miR-100, miR-21, miR-221, next miR-31, miR-143 and miR-23a were increased, whereas the levels of miR-217, miR-148a and miR-375 were decreased in the PDAC tissues (all p<0.05). Detailed data are available in Table 8. Table 8 Relative expression of miRNAs in PDAC compared with matched normal pancreatic tissue controls determined by qRT-PCR miRNA name Up-regulated PDAC N p-value Fold-change miR-155 5.56±1.00 2.71±0.66 <0.001 2.11±0.41 miR-100 7.40±2.21 3.91±1.32 <0.001 2.00±0.51 miR-21 3.80±0.99 1.7±0.35 <0.001 2.25±0.44 miR-221 8.03±2.77 3.26±0.67 <0.001 2.53±0.84 miR-31 6.52±0.98 2.93±0.39 <0.001 2.12±0.47 miR-143 7.45±1.22 2.21±1.43 <0.001 2.94±0.74 miR-23a 7.80±1.18 3.44±0.73 <0.001 2.