Apoptosis is a procedure used by higher organisms to keep homeostasis by removing cells that are excessively, broken, or potentially dangerous. Due to the increased degrees of procaspase 3 in cancer cells, the necessity of caspase 3 activation for apoptosis, and the relative downstream site of procaspase 3 within the apoptotic cascade, induction CTEP of apoptosis from the direct activation of procaspase 3 has been actively investigated as an individualized anticancer strategy. 8, 17 In 2006, the development of Procaspase Activating Compound 1 was reported. PAC 1 shows efficacy in numerous murine tumor models, induces apoptotic cell death in cancer cells, and enhances the enzymatic action of procaspase 3 in vitro. 8 Structureactivity relationship studies unmasked that the task of PAC 1 in vitro and in cell culture is dependent on the existence of the ortho hydroxy D acyl hydrazone moiety,18 a functional group known to participate in metal chelation. 19 Indeed, zinc can be a powerful inhibitor of the system and procaspase 3 enzymatic activity,20 by which PAC 1 stimulates procaspase 3 in vitro is through chelation of inhibitory zinc from procaspase 3, which allows Plastid procaspase 3 to process itself to the active form. 18, 20 This same basic process seems to be functional in cell culture as well: roughly a huge number of cellular zinc is not bound tightly but exists while the labile zinc pool. 21 As zinc from your labile pool has been found to co localize with procaspase 3,21 it appears that PAC 1 chelation of this labile zinc inside the cells increases procaspase 3 activity, ultimately causing apoptosis. PAC 1 may be safely administered to rats and study dogs at doses giving serum concentrations of 10 uM for 48-hours. 22 A sulfonamide containing derivative of PAC 1, named S PAC 1, could be safely given at doses that offer very high serum concentrations in rats. 23 Encouragingly, a veterinary clinical test of S PAC 1 in pet dogs with spontaneouslyoccurring lymphoma revealed this compound to become safe in most veterinary patients and good at reducing or stabilizing tumor development in 4 out of 6 patients. 23 This result provides proof principle for the idea that procaspase 3 service via little compound chelation Capecitabine molecular weight of labile zinc could be a safe and effective anti-cancer strategy. In the continuing search for stronger derivatives of PAC 1, we report herein the simultaneous synthesis of a combinatorial library of 837 PAC 1 analogues, the analysis of these materials for their power to induce demise of cancer cells in culture, and further characterization of six analogues of PAC 1 with enhanced effectiveness.