Loss of oxygen from the two position from substance 59 further decreased the anaerobic as well as aerobic activity emphasizing the importance of oxygen only at that position for both anaerobic as well as aerobic activity. Unsurprisingly, eliminating the side chain from 60 resulting in 61 also resulted in an inactive compound with this specific compound being significant as it is the 4 nitro isomer of metronidazole underscoring the importance of the position of the nitro group for the anaerobic action of metronidazole. The electron donating potential at the 2 position of the oxazine Fingolimod distributor band was found to be essential for activity since the replacement of the oxygen with carbon in 62 substantially affected both aerobic in addition to anaerobic activity with some recovery of anaerobic activity seen with the unsaturated species suggesting that SAR for aerobic and anaerobic activity are different and are determined by the electronics at this position. This idea was further supported by the observation that replacement of the 2 position air with electron donating groups, such as nitrogen or sulfur, had no effect on the aerobic activity but decreased anaerobic potency, while replacement with electron withdrawing groups dramatically reduced or abrogated aerobic Plastid activity without much effect on anaerobic activity. The fact that the benzyloxybenzyloxy substituent on the band creates an element more active than PA 824 suggested the existence of a larger hydrophobic pocket near the active site of the enzyme that interacts with the drug. To discover the level of this hydrophobic pocket, SAR of the end of PA 824 was examined. The situation of solubility of these compounds with the extra hydrophobe was removed by substituting the ether analog with the corresponding amine analog where the aminoderivatives of PA 824 and 49 produced compounds 70 and 71 with slightly enhanced activity. On raising the linker dimension connecting the 6 position amine with the trifuoromethoxybenzene fragrant moiety from two to four carbons, the aerobic activity was found to sequentially enhance with aerobic activity achieving a maximum with the aminobutyl 824, although the 5 carbon linker in aminopentyl 824 had reduced activity. There was no significant improvement of the anaerobic supplier Capecitabine activity on changing the linker size, indicating a SAR for aerobic and anaerobic activity with respect to the hydrophobic tail area of the drug. Further exploration of the hydrophobic binding pocket was undertaken with o, m and plinked biphenyl analogs mounted on the nitroimidazooxazine via ether linkage. The o linked biphenyls showed exercise, followed closely by the m linked analogs, as the p linked biphenyl analogs were probably the most effective. The game pattern did not alter somewhat with substitutions in the 2nd aryl ring. This suggested that the hydrophobic pocket is just about linear with average tolerability round the terminus of the next aryl ring.