1.5. The experimental protocols 2.1.5.1. Characterization Irbesartan RAAS inhibitor of neurons. The recording electrode was advanced through the spinal cord and the receptive field was evaluated in all three trigeminal territories. The first step was to obtain sensitive mater identifya neuron to stimulation of the ophthalmic trigeminal dermatome to convergent input from dura. Three answers stable reference for the stimulation perimiddle meningeal artery and dural cutaneous receptive field stimulation were then recorded. The parameters of stimulation and recording, and then remained w During the experiment constant. Evoked Peristimulus histograms were used to observe the fire in response to a beautiful dlichen and harmless cutaneous stimulation electrodes of the ophthalmic dermatome and post-stimulus histograms were used to indicate units firing in response to electrical stimulation dura. 2.1.5.2. A11 block L Sion and Drug Administration. The core was injured electrically ipsilateral A11, and the effect observed on evoked firing in the TCC claim 5-minute intervals for 15 minutes, after which one of the above mentioned Hnten drugs or salt solutions Was intravenously administered solution vehicle S, and evoked firing from 20 to 40 minutes observed postlesioning. 2.1.6. The cells of the data analysis with a latency of 20 ms post-stimulation have 5 shots are classified as activated by the input fiber of the ads. At the beginning of each experiment, three images consistent baseline firing by trigeminal stimulation of the dura mater and meningeal perimiddle cutaneous receptive fields were produced. All of the following data were normalized and expressed as percentage of baseline averaged. Some data were also normalized to the L Sion reactions, but the predrug buy Cinacalcet interventions, in order to compare these responses. Analysis of variance for repeated measures was applied to the data with L A11 Performed sion or pharmacological intervention. Post-hoc Bonferroni correction for multiple comparisons was used fa Is routine. If Mauchly testSph Rizit t was injured, adjusted degrees of freedom w Greenhouse Geisser are reported. In some cases F Analysis of variance with repeated measures between subjects factor and group quinpirole compared to other drug groups was carried out. Student paired t-test with post hoc correction was used to test individual time points are important to the average of three prelesion baselines for comparison, and all data are expressed as mean ± SEM. Significance was set at P 0.05. 2.1.7. Post operative examination of tissue samples in all experiments, the recording site by the CCI L mission Immediately prior to terminal Anesthesiology marks. The brain was removed and coronal sections were cut 60 lm thick and mounted slides and gegengef Rbt with cresyl violet dye before they viewed under a microscope. With the rat brain MDV3100 atlas as a reference, a brand identified L Sion regions of both the recording electrode in the TCC and the stimulating electrode into the A11. 2.2. Immunohistofluorescence characterization of D2 like receptors and 5-receptors in the HT1B/1D 2.2.1 trigeminocervical complex. Perfusion and fixation of male pattern Sprague Dawley rats weighing 300 g were deeply in Sthesiert with sodium pentobarbital and transcardially with heparinized saline 1% Perfused with 4% formaldehyde solution, followed.